Expression of membrane-type 5 matrix metalloproteinase in human endometrium and endometriosis.

Expression of membrane-type 5 matrix metalloproteinase in human endometrium and endometriosis. Research Abstract Details 

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Expression of membrane-type 5 matrix metalloproteinase in human endometrium and endometriosis. Abstract Text:

Regine Gaetje,Uwe Holtrich,Knut Engels,Katherina Kourtis,Eva Cikrit,Stefan Kissler,Achim Rody,Thomas Karn,Manfred Kaufmann,Regine Gaetje,Uwe Holtrich,Knut Engels,Katherina Kourtis,Eva Cikrit,Stefan Kissler,Achim Rody,Thomas Karn,Manfred Kaufmann,

BACKGROUND: The metalloproteinases (MMPs) are a family of proteolytic enzymes involved in tissue remodeling and cell migration. Endometrial tissue remodeling proceeds during the menstrual cycle and requires a temporary and spatially balanced expression of several different MMPs. Various members of the MMPs also seem to play an important role in the invasion process of endometriosis; however, so far only a limited number of studies have focused on membrane-associated MMPs. METHODS: The present study investigated the expression of membrane-type 5 metalloproteinase (MT5-MMP) in the human endometrium and endometriotic lesions by microarray hybridization, real-time polymerase chain reaction (PCR) and immunofluorescence. RESULTS: Both the gene chip expression analyses as well as PCR indicated expression of MT5-MMP in normal human endometrium and strongly elevated transcript levels in most peritoneal endometriosis lesions analyzed. Moreover we detected enhanced MT5-MMP expression in the eutopic endometrium from patients suffering from endometriosis, further supporting a role of MT5-MMP in the formation of endometriosis. Immunohistochemical analysis was used to determine the intracellular localization and tissue distribution of MT5-MMP. While the MT5-MMP antigen expression could be clearly attributed to the membrane of epithelial cells, a highly complex differential immunohistochemical staining of MT5-MMP in the various compartments of endometrial tissue was observed. The strongest staining was seen in luminal epithelial cells, whereas endometrial glands frequently showed partial expression of MT5-MMP. CONCLUSION: Our microarray analysis and real-time PCR of MT5-MMP transcripts may point to an elevated tissue remodeling and cell migration in endometrium from endometriosis patients as implied by the function of related MMPs.

Expression of membrane-type 5 matrix metalloproteinase in human endometrium and endometriosis. Publishing Authors By Initials

R Gaetje,U Holtrich,K Engels,K Kourtis,E Cikrit,S Kissler,A Rody,T Karn,M Kaufmann,R Gaetje,U Holtrich,K Engels,K Kourtis,E Cikrit,S Kissler,A Rody,T Karn,M Kaufmann,

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Expression of membrane-type 5 matrix metalloproteinase in human endometrium and endometriosis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Gynecological endocrinology : the official journal

VOLUME: 23

Page Numbers: 567-73

Journal Abbreviation: Gynecol. Endocrinol.

ISSN: 0951-3590

DAY: 22

MONTH: Oct

YEAR: 2007

Expression of membrane-type 5 matrix metalloproteinase in human endometrium and endometriosis. Information

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LANGUAGE: eng

NlmUniqueID: 8807913

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AFFILIATION: Department of Obstetrics and Gynecology, Johann Wolfgang Goethe-University, Frankfurt, Germany. gaetje@em.uni-frankfurt.de

Country: England

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MEDLINETA: Gynecol Endocrinol

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