Expression of matrix metalloproteinases 2 and 9 in experimental corneal injury and fungal keratitis.

Expression of matrix metalloproteinases 2 and 9 in experimental corneal injury and fungal keratitis. Research Abstract Details 

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Expression of matrix metalloproteinases 2 and 9 in experimental corneal injury and fungal keratitis. Abstract Text:

Bradley M Mitchell,Tzu G Wu,Eva-Marie Chong,J Carey Pate,Kirk R Wilhelmus,

PURPOSE: Levels of matrix metalloproteinases (MMPs) can be modulated during corneal infection, but little is known about MMP profiles during fungal keratitis. The purpose of this study was to determine the effect of corneal trauma and immunosuppressive treatment on the expression kinetics of MMP-2 and MMP-9 during experimental keratomycosis. METHODS: Corneas of immunocompetent and cyclophosphamide-treated adult BALB/c mice were topically inoculated with 1 x 10 culturable units of Fusarium solani or mock-inoculated with or without superficial corneal scarification. Eyes were scored daily for disease severity and processed for zymography after 1.5 hours, 6 hours, 1 day, 4 days, or 8 days. Gelatinase activity was densitometrically quantitated and normalized to MMP-2 and MMP-9 controls. RESULTS: MMP-9 levels in nontraumatized eyes transiently increased at 6 hours after fungal exposure, but this increase was inhibited by cyclophosphamide treatment. Corneal injury significantly induced early MMP-9 expression that returned to baseline levels within 4 days. Cyclophosphamide pretreatment reduced and delayed MMP-9 after scarification. Fusarium exposure dampened the MMP-9 response to corneal trauma in immunocompetent and cyclophosphamide-treated animals. Ocular levels of MMP-2 were not affected by scarification, fungal exposure, or immunosuppressive treatment. CONCLUSIONS: Ocular MMP-9 levels, but not MMP-2 levels, increased soon after corneal injury. A similar, although muted, MMP-9 response occurs during early filamentous fungal keratitis, with a kinetic profile similar to corneal disease progression. The early stage of ulcerative keratitis may involve selective regulation of corneal matrix metalloproteinases, suggesting an initial opportunity for therapeutic intervention.

Expression of matrix metalloproteinases 2 and 9 in experimental corneal injury and fungal keratitis. Publishing Authors By Initials

BM Mitchell,TG Wu,EM Chong,JC Pate,KR Wilhelmus,

For similar disorders of environmental origin: wounds and injuries: wounds, nonpenetrating research abstracts see: disorders of environmental origin: wounds and injuries: wounds, nonpenetrating research

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Expression of matrix metalloproteinases 2 and 9 in experimental corneal injury and fungal keratitis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Cornea

VOLUME: 26

Page Numbers: 589-93

Journal Abbreviation: Cornea

ISSN: 0277-3740

DAY: 3

MONTH: Jun

YEAR: 2007

Expression of matrix metalloproteinases 2 and 9 in experimental corneal injury and fungal keratitis. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8216186

Expression of matrix metalloproteinases 2 and 9 in experimental corneal injury and fungal keratitis. Keywords Mesh Terms:

KEYWORDS: Wounds, Nonpenetrating

MESH TERMS: microbiology

Chemical & Substance for Abstract: Expression of matrix metalloproteinases 2 and 9 in experimental corneal injury and fungal keratitis. Information

Substance Name: Matrix Metalloproteinase 9

Registry Number: EC 3.4.24.35

Grant and Affiliation Information for Expression of matrix metalloproteinases 2 and 9 in experimental corneal injury and fungal keratitis.

AFFILIATION: Sid W. Richardson Ocular Microbiology Laboratory, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, TX 77030, USA. bmm@bcm.tmc.edu

Country: United States

AGENCY: United States NEI

GRANT: R03 EY13782

ACRONYM: EY

MEDLINETA: Cornea

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