[Expression of IL-23 gene in murine colon carcinoma cells transduced with recombinant retrovirus and its anti-tumor activity]

[Expression of IL-23 gene in murine colon carcinoma cells transduced with recombinant retrovirus and its anti-tumor activity] Research Abstract Details 

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[Expression of IL-23 gene in murine colon carcinoma cells transduced with recombinant retrovirus and its anti-tumor activity] Abstract Text:

Jing-sheng Hao,Bao-en Shan,Qiao-xia Li,Qi-liang Li,Xiao-mei Fu,

AIM: To obtain murine colon carcinoma cell lines stably transducted by recombinant retrovirus encoding mIL-23 gene. METHODS: The retrovirus vector was used to transduce the mIL-23 gene into murine colon carcinoma cells (Colon26) and stable clones expressing mIL-23 (Colon26/IL-23) were obtained by screening with G418. The expression of mIL-23 gene was detected by PCR and RT-PCR. The amount of mIL-23 secreted from Colon26/IL-23 cells and IFN-gamma production by murine splenocytes induced by mIL-23 were determined by ELISA. MTT colorimetry was used to assess the in vitro growth of Colon26/IL-23 cells. BALB/c mice were injected s.c. with Colon26/IL-23 cells to observe their tumorigenicity. RESULTS: Colon26/IL-23 cells were set up. Colon26/IL-23 could secrete mIL-23 which could induce the IFN-gamma production by murine splenocytes. The growth of Colon26/IL-23 cells was similar to that of Colon26 cells in vitro. But the tumorigenicity of Colon26/IL-23 cells was lower than that of Colon26 cells in vivo. CONCLUSION: Colon26/IL-23 cells secreting mIL-23 were obtained and IL-23 had anti-tumor activity.

[Expression of IL-23 gene in murine colon carcinoma cells transduced with recombinant retrovirus and its anti-tumor activity] Publishing Authors By Initials

JS Hao,BE Shan,QX Li,QL Li,XM Fu,

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[Expression of IL-23 gene in murine colon carcinoma cells transduced with recombinant retrovirus and its anti-tumor activity] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese jour

VOLUME: 21

Page Numbers: 226-8, 232

Journal Abbreviation: Xi Bao Yu Fen Zi Mian Yi Xue Z

ISSN: 1007-8738

DAY: 15

MONTH: Mar

YEAR: 2005

[Expression of IL-23 gene in murine colon carcinoma cells transduced with recombinant retrovirus and its anti-tumor activity] Information

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LANGUAGE: chi

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AFFILIATION: Research Center, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China. jshhao@yahoo.com.cn

Country: China

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MEDLINETA: Xi Bao Yu Fen Zi Mian Yi Xue Z

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