Expression of human alpha-lactalbumin in transgenic tobacco.

Expression of human alpha-lactalbumin in transgenic tobacco. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Expression of human alpha-lactalbumin in transgenic tobacco. Abstract Text:

K Takase,K Hagiwara,

alphaLA-Lactalbumin (alphaLA), a major milk protein, is the regulatory subunit of lactose synthase. To assess the production of recombinant alphaLA in plants, the cDNAs for human alphaLA with or without its own signal sequence were introduced into tobacco plants under the control of the cauliflower mosaic virus 35S promoter.The gene integration and expression at the mRNA level were confirmed in several regenerated plants, while the expression at the protein level could be confirmed only in a transgenic tobacco transformed with the gene containing the signal sequence. The tobacco-expressed alphaLA migrated in SDS-PAGE with identical mobility to alphaLA prepared from human milk, indicating that the signal peptide of human alphaLA was correctly processed to yield a mature protein in tobacco plants. The expressed alphaLA (ca. 5 microg/g of fresh leaves) was found in the soluble fraction and eluted from a DEAE-Sepharose column in the same salt concentration range as the milk alphaLA. The partially purified tobacco-alphaLA was fully active in the synthesis of lactose when combined with galactosyltransferase. Thus, the transgenic tobacco produces a fully active mature alphaLA in a soluble form.

Expression of human alpha-lactalbumin in transgenic tobacco. Publishing Authors By Initials

K Takase,K Hagiwara,

For similar genetic processes: recombination, genetic: transformation, genetic research abstracts see: genetic processes: recombination, genetic: transformation, genetic research

PUBMED ID PMID:

MEDLINE DATE:

Expression of human alpha-lactalbumin in transgenic tobacco. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 123

Page Numbers: 440-4

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Mar

YEAR: 1998

Expression of human alpha-lactalbumin in transgenic tobacco. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Expression of human alpha-lactalbumin in transgenic tobacco. Keywords Mesh Terms:

KEYWORDS: Transformation, Genetic

MESH TERMS: genetics

Chemical & Substance for Abstract: Expression of human alpha-lactalbumin in transgenic tobacco. Information

Substance Name: Lactalbumin

Registry Number: 9013-90-5

Grant and Affiliation Information for Expression of human alpha-lactalbumin in transgenic tobacco.

AFFILIATION: Department of Biotechnology, National Institute of Agrobiological Resources, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan. ktakase@abr.affrc.go.jp

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Expression of human alpha-lactalbumin in transgenic tobacco Related Publications

• A simple and efficient carrier for porcelain laminate veneers.
• An ultra fast, radiation-resistant fast neutron detector.
• Biphenylene-substituted ruthenocenylphosphine for suzuki-miyaura coupling of aryl chlorides.
• Concentration of mRNA for the natriuretic peptide receptor-C in hypertrophic chondrocytes of the fetal mouse tibia.
• Correlation between induction of expression of biglycan and mineralization by C-type natriuretic peptide in osteoblastic cells.
• Expression of human alpha-lactalbumin in transgenic tobacco.
• His145-Trp146 residues and the disulfide-linked loops in atrial natriuretic peptide receptor are critical for the ligand-binding activity.
• Inclusion bodies of the vaccinia virus.
• Localization and characterization of a novel receptor for endothelin in the gills of the rainbow trout.
• Peptide nucleic acid-locked nucleic acid polymerase chain reaction clamp-based detection test for gefitinib-refractory T790M epidermal growth factor receptor mutation.
• STUDIES ON THE SPLIT MEASUREMENT OF THE DLCO, VC AND DM BY PLOTTING METHOD IN NORMAL AND SURGICAL DISEASES.
• The Fox-1 family and SUP-12 coordinately regulate tissue-specific alternative splicing in vivo.
• The diagnostic application of ultrasound to the disease in mediastinal organs. Ultrasono-tomography for the heart and great vessels (2).
• Total synthesis of the hallucinogenic neoclerodane diterpenoid salvinorin a.
• Transgenic expression of VpreB-3 under the control of the immunoglobulin heavy chain enhancer and SV40 promoter.
• Traumatic intramural hematoma of duodenum with thrombasthenia in childhood.
• Treatment of myoblastic C2C12 cells with BMP-2 stimulates vitamin D-induced formation of osteoclasts.
• Two circular chromosomes of unequal copy number make up the mitochondrial genome of the rotifer Brachionus plicatilis.
• Why are attributions to discrimination interpersonally costly? A test of system- and group-justifying motivations.
• [A case report of surgical treatment for aortic valve papillary fibroelastoma]
• [Bilateral simultaneous thoracotomy for unilateral spontaneous pneumothorax, with special reference to the operative indication considered from its contralateral occurrence rate]
• [Changing nursing duty and work analysis. Need for analysis of the modern nursing duty]
• [Effect of intravenous injection of oleandomycin on gynecological infections]
• [Methods in nursing surveys. 9. Nursing surveys. Methodology. II. A survey on nursing structures and the nursing teaching staff]
• [Methods of nursing surveys. 10. Nursing surveys: research methods. IV. Surveys on nursing education]
• [Myocardial protection during aorta-coronary bypass surgery--from the viewpoint of sequence of vein graft anastomosis]
• [Pulmonary infiltration with eosinophilia, eosinophilic pneumonia]
• [Studies on the use of priming blood after extracorporeal circulation - analysis on "Haemonetics Cell Saver" (author's transl)]
• [The work of nurses in the high-technology medical care and formation of nursing skills]
• [Thymic cyst--a case report and review of literature]