Expression of g protein-coupled receptor-30, a g protein-coupled membrane estrogen receptor, in oxytocin neurons of the rat paraventricular and supraoptic nuclei.

Expression of g protein-coupled receptor-30, a g protein-coupled membrane estrogen receptor, in oxytocin neurons of the rat paraventricular and supraoptic nuclei. Research Abstract Details 

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Expression of g protein-coupled receptor-30, a g protein-coupled membrane estrogen receptor, in oxytocin neurons of the rat paraventricular and supraoptic nuclei. Abstract Text:

Hirotaka Sakamoto,Ken-Ichi Matsuda,Koji Hosokawa,Mayumi Nishi,John F Morris,Eric R Prossnitz,Mitsuhiro Kawata,Hirotaka Sakamoto,Ken-ichi Matsuda,Koji Hosokawa,Mayumi Nishi,John F Morris,Eric R Prossnitz,Mitsuhiro Kawata,

The regulatory actions of estrogens on magnocellular oxytocin (OT) neurons of the paraventricular and supraoptic nuclei are well documented. Although the expression and distribution of nuclear estrogen receptor-beta, but not estrogen receptor-alpha, in the OT neuron has been described, the nuclear receptors may not explain all aspects of estrogen function in the hypothalamic OT neuron. Recently a G protein-coupled receptor (GPR) for estrogens, GPR30, has been identified as a membrane-localized estrogen receptor in several cancer cell lines. In this study, we therefore investigated the expression and localization of GPR30 in magnocellular OT neurons to understand the mode of rapid estrogen actions within these neurons. Here we show that, in the paraventricular nucleus and supraoptic nucleus, GPR30 is expressed in magnocellular OT neurons at both mRNA and protein levels but is not expressed in vasopressin neurons. Specific markers for intracellular organelles and immunoelectron microscopy revealed that GPR30 was localized mainly in the Golgi apparatus of the neurons but could not be detected at the cell surface. In addition, the expression of GPR30 is also detected in the neurohypophysis. These results suggest that GPR30 may serve primarily as a nongenomic transducer of estrogen actions in the hypothalamo-neurohypophyseal system.

Expression of g protein-coupled receptor-30, a g protein-coupled membrane estrogen receptor, in oxytocin neurons of the rat paraventricular and supraoptic nuclei. Publishing Authors By Initials

H Sakamoto,K Matsuda,K Hosokawa,M Nishi,JF Morris,ER Prossnitz,M Kawata,H Sakamoto,K Matsuda,K Hosokawa,M Nishi,JF Morris,ER Prossnitz,M Kawata,

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Expression of g protein-coupled receptor-30, a g protein-coupled membrane estrogen receptor, in oxytocin neurons of the rat paraventricular and supraoptic nuclei. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Endocrinology

VOLUME: 148

Page Numbers: 5842-50

Journal Abbreviation: Endocrinology

ISSN: 0013-7227

DAY: 13

MONTH: 09

YEAR: 2007

Expression of g protein-coupled receptor-30, a g protein-coupled membrane estrogen receptor, in oxytocin neurons of the rat paraventricular and supraoptic nuclei. Information

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LANGUAGE: eng

NlmUniqueID: 375040

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Grant and Affiliation Information for Expression of g protein-coupled receptor-30, a g protein-coupled membrane estrogen receptor, in oxytocin neurons of the rat paraventricular and supraoptic nuclei.

AFFILIATION: Department of Anatomy and Neurobiology, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan. hsakamo@koto.kpu-m.ac.jp.

Country: United States

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MEDLINETA: Endocrinology

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