Expression of extracellular matrix metalloproteinase inducer and matrix metalloproteinases during mouse embryonic development.

Expression of extracellular matrix metalloproteinase inducer and matrix metalloproteinases during mouse embryonic development. Research Abstract Details 

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Expression of extracellular matrix metalloproteinase inducer and matrix metalloproteinases during mouse embryonic development. Abstract Text:

Li Chen,Masaaki Nakai,Robert J Belton,Romana A Nowak,

Mouse embryo implantation is a highly invasive and controlled process that involves remodeling and degradation of the extracellular matrix of the uterus. Matrix metalloproteinases (MMPs) are the main proteinases facilitating this process. Extracellular matrix metalloproteinase inducer (EMMPRIN) can stimulate the production of MMPs and is required for successful implantation in the mouse. The aims of the present study were to examine the expression profiles of mRNA and proteins for EMMPRIN and MMPs in the developing mouse embryo in vitro, and to study whether EMMPRIN protein induces the production of MMPs by mouse blastocysts. EMMPRIN mRNA, detected by RT-PCR, was present at all stages of embryo development from the one-cell to the blastocyst outgrowth. EMMPRIN protein, observed by confocal microscopy, was present on the cell surface at the same stages of development as was the mRNA. Of seven MMPs studied, murine collagenase-like A (Mcol-A), murine collagenase-like B (Mcol-B) and gelatinase A (MMP-2) mRNAs were detected only in blastocyst outgrowths by RT-PCR. Gelatinase B (MMP-9) mRNA was detected both in expanded blastocysts and blastocyst outgrowths. MMP-2 and -9 proteins were detected in the cytoplasm of outgrowing trophoblast cells. Collagenase-2 (MMP-8), collagenase-3 (MMP-13), or stromelysin-1 (MMP-3) mRNAs were not present at any stage of pre- or peri-implantation mouse embryo development. Quantitative RT-PCR analyses showed that recombinant EMMPRIN protein did not stimulate MMP-2 or -9 expression by mouse blastocyst outgrowths. These data suggest that EMMPRIN may regulate physiological functions other than MMP production by mouse embryos during implantation.

Expression of extracellular matrix metalloproteinase inducer and matrix metalloproteinases during mouse embryonic development. Publishing Authors By Initials

L Chen,M Nakai,RJ Belton,RA Nowak,

For similar cells: trophoblasts research abstracts see: cells: trophoblasts research

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Expression of extracellular matrix metalloproteinase inducer and matrix metalloproteinases during mouse embryonic development. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Reproduction (Cambridge, England)

VOLUME: 133

Page Numbers: 405-14

Journal Abbreviation: Reproduction

ISSN: 1470-1626

DAY: 3

MONTH: Feb

YEAR: 2007

Expression of extracellular matrix metalloproteinase inducer and matrix metalloproteinases during mouse embryonic development. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100966036

Expression of extracellular matrix metalloproteinase inducer and matrix metalloproteinases during mouse embryonic development. Keywords Mesh Terms:

KEYWORDS: Trophoblasts

MESH TERMS: metabolism

Chemical & Substance for Abstract: Expression of extracellular matrix metalloproteinase inducer and matrix metalloproteinases during mouse embryonic development. Information

Substance Name: Matrix Metalloproteinase 9

Registry Number: EC 3.4.24.35

Grant and Affiliation Information for Expression of extracellular matrix metalloproteinase inducer and matrix metalloproteinases during mouse embryonic development.

AFFILIATION: Department of Animal Sciences, University of Illinois, Urbana, Illinois 61801, USA.

Country: England

AGENCY: United States NICHD

GRANT: U54 HD40093

ACRONYM: HD

MEDLINETA: Reproduction

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