Expression of cytokeratin 19 in the diagnosis of thyroid papillary carcinoma by quantitative polymerase chain reaction.

Expression of cytokeratin 19 in the diagnosis of thyroid papillary carcinoma by quantitative polymerase chain reaction. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Expression of cytokeratin 19 in the diagnosis of thyroid papillary carcinoma by quantitative polymerase chain reaction. Abstract Text:

OBJECTIVE: To examine cytokeratin 19 (CK19) expression levels by immunostaining for protein and quantitative reverse-transcription polymerase chain reaction (qPCR) for messenger RNA in thyroid surgical specimens from patients with papillary carcinoma (PC) and other types of thyroid lesions. METHODS: A total of 54 randomly selected postoperative thyroid tissue samples were collected for formalin-fixed paraffin-embedded sectioning or flash-frozen total RNA extraction for complementary DNA synthesis (or both). Tissue sections were stained for CK19 expression with use of a specific monoclonal antibody. qPCR was performed on synthesized complementary DNA with sequence-specific primers for human CK19 in conjunction with ribonucleoprotein S18 for normalization. RESULTS: CK19 immunostaining was diffuse and intense in all PC lesions and considerably less in specimens that harbored both Hashimoto thyroiditis (HT) and PC. CK19 immunostaining was mostly absent in areas of multinodular goiter (MNG), with occasional focal staining. HT and Hürthle cell adenoma were essentially negative for CK19 immunostaining, except for weak staining in focal areas. Analysis of CK19 gene expression by qPCR revealed that the PC samples tested (n = 21) had significantly higher levels in comparison with all other groups (P < 0.0001). Furthermore, PC had a 32-fold mean level increase in CK19 expression in comparison with CK19 expression in MNG. Hürthle cell adenoma (n = 5) and HT (n = 7) lesions were similar to MNG in CK19 expression, with some overlap of CK19 between HT and PC. CONCLUSION: The data indicate that expression of CK19 by qPCR is a quantitative method for distinguishing PC lesions from other types of thyroid lesions, in contrast to the more qualitative immunohistochemistry. Moreover, qPCR of CK19 is a valid method that could be used as an ancillary tool in diagnosing thyroid cancer. The expression of CK19 by qPCR may be adopted, in combination with other markers, for molecular definition of the various subtypes of thyroid lesions assessed by fine-needle aspiration biopsy in the preoperative diagnosis of thyroid lesions.

Expression of cytokeratin 19 in the diagnosis of thyroid papillary carcinoma by quantitative polymerase chain reaction. Publishing Authors By Initials

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Expression of cytokeratin 19 in the diagnosis of thyroid papillary carcinoma by quantitative polymerase chain reaction. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Endocrine practice : official journal of the Ameri

VOLUME: 14

Page Numbers: 168-74

Journal Abbreviation:

ISSN: 1934-2403

DAY: 29

MONTH: Mar

YEAR: 2008

Expression of cytokeratin 19 in the diagnosis of thyroid papillary carcinoma by quantitative polymerase chain reaction. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9607439

Expression of cytokeratin 19 in the diagnosis of thyroid papillary carcinoma by quantitative polymerase chain reaction. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Expression of cytokeratin 19 in the diagnosis of thyroid papillary carcinoma by quantitative polymerase chain reaction. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Expression of cytokeratin 19 in the diagnosis of thyroid papillary carcinoma by quantitative polymerase chain reaction.

AFFILIATION: Department of Medicine, Section of Endocrinology, Diabetes and Nutrition, Boston University School of Medicine, Boston, Massachusetts 02118-2308, USA.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Endocr Pract

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Expression of cytokeratin 19 in the diagnosis of thyroid papillary carcinoma by quantitative polymerase chain reaction Related Publications

• "Cutaneous manifestations of internal malignant tumors" by Becker, Kahn and Rothman, June 1942. Commentary: Migratory necrolytic erythema.
• Assessment of thyroid function and urinary and breast milk iodine concentrations in healthy newborns and their mothers in Tehran.
• Association of first-trimester thyroid function test values with thyroperoxidase antibody status, smoking, and multivitamin use.
• Basal cell nevus syndrome with inflammatory disease of the bowel.
• Breast milk iodine and perchlorate concentrations in lactating Boston-area women.
• Clinical and molecular analysis of arylsulfatase E in patients with brachytelephalangic chondrodysplasia punctata.
• Clinical studies of exposure to perchlorate in the United States.
• Computers and morphology.
• Effects of iodine on thyroid function in man.
• Ethical conflicts in long-term care of the aged.
• Evidence that high calcium and vitamin D intake decrease the risk of breast cancer in premenopausal women: implications for breast cancer prevention and screening.
• Expression of cytokeratin 19 in the diagnosis of thyroid papillary carcinoma by quantitative polymerase chain reaction.
• Graduate training in dermatology. opinions of those in training 1968.
• Impaired venous hemodynamics in a minority of patients with chronic leg ulcers due to sickle cell anemia.
• Interval percutaneous cholecystostomy is effective for decompression of the common bile duct in high-risk elderly patients prior to endoscopic retrograde cholangiopancreatography.
• National Health and Nutrition Examination Survey III thyroid-stimulating hormone (TSH)-thyroperoxidase antibody relationships demonstrate that TSH upper reference limits may be skewed by occult thyroid dysfunction.
• Predilection of hemolytic anemia-associated leg ulcers for the medial malleolus.
• Prednisone treatment for vestibular neuritis.
• Reproducible measurements to quantify cutaneous involvement in scleroderma.
• The effect of type of delivery and povidone-iodine application at delivery on cord dried-blood-specimen thyrotropin level and the rate of hyperthyrotropinemia in mature and normal-birth-weight neonates residing in an iodine-replete area: report of tehran
• The school system as the vehicle of preventive psychiatry.
• The use of immunohistologic analysis in differentiating cutaneous T-cell lymphoma from psoriasis and dermatitis.
• The use of the thematic apperception test in psychotherapy.
• Thyroid function and lipid subparticle sizes in patients with short-term hypothyroidism and a population-based cohort.
• Transient congenital hypothyroidism in an iodine-replete area is not related to parental consanguinity, mode of delivery, goitrogens, iodine exposure, or thyrotropin receptor autoantibodies.
• Use of methotrexate to treat isolated Graves ophthalmopathy developing years after thyroidectomy and iodine 131 treatment of papillary thyroid cancer.
• [Treatment of acute Chagas-Mazza disease]