Expression of Clostridium acetobutylicum butanol synthetic genes in Escherichia coli.

Expression of Clostridium acetobutylicum butanol synthetic genes in Escherichia coli. Research Abstract Details 

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Expression of Clostridium acetobutylicum butanol synthetic genes in Escherichia coli. Abstract Text:

Masayuki Inui,Masako Suda,Sakurako Kimura,Kaori Yasuda,Hiroaki Suzuki,Hiroshi Toda,Shogo Yamamoto,Shohei Okino,Nobuaki Suzuki,Hideaki Yukawa,

A recombinant butanol pathway composed of Clostridium acetobutylicum ATCC 824 genes, thiL, hbd, crt, bcd-etfB-etfA, and adhe1 (or adhe) coding for acetyl-CoA acetyltransferase (THL), beta-hydroxybutyryl-CoA dehydrogenase (HBD), 3-hydroxybutyryl-CoA dehydratase (CRT), butyryl-CoA dehydrogenase (BCD), butyraldehyde dehydrogenase (BYDH), and butanol dehydrogenase (BDH), under the tac promoter control was constructed and was introduced into Escherichia coli. The functional expression of these six enzymes was proved by demonstrating the corresponding enzyme activities using spectrophotometric, high performance liquid chromatography and gas chromatography analyses. The BCD activity, which was not detected in E. coli previously, was shown in the present study by performing the procedure from cell extract preparation to activity measurement under anaerobic condition. Moreover, the etfA and etfB co-expression was found to be essential for the BCD activity. In the case of BYDH activity, the adhe gene product was shown to have higher specificity towards butyryl-CoA compared to the adhe1 product. Butanol production from glucose was achieved by the highly concentrated cells of the butanologenic E. coli strains, BUT1 with adhe1 and BUT2 with adhe, under anaerobic condition, and the BUT1 and BUT2 strains were shown to produce 4 and 16-mM butanol with 6- and 1-mM butyrate as a byproduct, respectively. This study reports the novel butanol production by an aerobically pregrown microorganism possessing the genes of a strict anaerobe, Clostridium acetobutylicum.

Expression of Clostridium acetobutylicum butanol synthetic genes in Escherichia coli. Publishing Authors By Initials

M Inui,M Suda,S Kimura,K Yasuda,H Suzuki,H Toda,S Yamamoto,S Okino,N Suzuki,H Yukawa,

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Expression of Clostridium acetobutylicum butanol synthetic genes in Escherichia coli. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Applied microbiology and biotechnology

VOLUME: 77

Page Numbers: 1305-16

Journal Abbreviation: Appl. Microbiol. Biotechnol.

ISSN: 0175-7598

DAY: 1

MONTH: 12

YEAR: 2007

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LANGUAGE: eng

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Grant and Affiliation Information for Expression of Clostridium acetobutylicum butanol synthetic genes in Escherichia coli.

AFFILIATION: Research Institute of Innovative Technology for the Earth, 9-2 Kizugawadai, Kizugawa, Kyoto, 619-0292, Japan, mmg-lab@rite.or.jp.

Country: Germany

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MEDLINETA: Appl Microbiol Biotechnol

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