Expression of cDNA for batroxobin, a thrombin-like snake venom enzyme.

Expression of cDNA for batroxobin, a thrombin-like snake venom enzyme. Research Abstract Details 

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Expression of cDNA for batroxobin, a thrombin-like snake venom enzyme. Abstract Text:

M Maeda,S Satoh,S Suzuki,M Niwa,N Itoh,I Yamashina,

The cloned cDNA for batroxobin has been expressed in E. coli. Batroxobin could only be obtained as intracellular aggregates of fusion proteins, fused with a small peptide. To obtain the mature batroxobin, the recognition sequence for thrombin was inserted between the peptide and the mature batroxobin. This fusion protein accumulated in an insoluble form and could easily be purified. After site-specific cleavage of the fusion protein with thrombin, recombinant batroxobin was isolated by preparative electrophoresis. Batroxobin with enzymatic activity was obtained by the refolding of recombinant batroxobin.

Expression of cDNA for batroxobin, a thrombin-like snake venom enzyme. Publishing Authors By Initials

M Maeda,S Satoh,S Suzuki,M Niwa,N Itoh,I Yamashina,

For similar enzymes and coenzymes: enzymes: hydrolases: peptide hydrolases: endopeptidases: serine endopeptidases: thrombin research abstracts see: enzymes and coenzymes: enzymes: hydrolases: peptide hydrolases: endopeptidases: serine endopeptidases: thrombin research

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Expression of cDNA for batroxobin, a thrombin-like snake venom enzyme. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 109

Page Numbers: 632-7

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Apr

YEAR: 1991

Expression of cDNA for batroxobin, a thrombin-like snake venom enzyme. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Expression of cDNA for batroxobin, a thrombin-like snake venom enzyme. Keywords Mesh Terms:

KEYWORDS: Thrombin

MESH TERMS: metabolism

Chemical & Substance for Abstract: Expression of cDNA for batroxobin, a thrombin-like snake venom enzyme. Information

Substance Name: Thrombin

Registry Number: EC 3.4.21.5

Grant and Affiliation Information for Expression of cDNA for batroxobin, a thrombin-like snake venom enzyme.

AFFILIATION: Product Development Laboratories, Fujisawa Pharmaceutical Co., Ltd., Osaka.

Country: JAPAN

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MEDLINETA: J Biochem

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