Expression of cathepsin P mRNA, protein and activity in the rat choriocarcinoma cell line, Rcho-1, during giant cell transformation.

Expression of cathepsin P mRNA, protein and activity in the rat choriocarcinoma cell line, Rcho-1, during giant cell transformation. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Expression of cathepsin P mRNA, protein and activity in the rat choriocarcinoma cell line, Rcho-1, during giant cell transformation. Abstract Text:

M Hassanein,B D Korant,G Lu,R W Mason,

Lysosomal proteases perform critical functions in protein turnover and are essential for normal growth and development. Cathepsin P is a member of a newly discovered family of lysosomal cysteine proteases uniquely expressed in rodent placenta (PECs), and is closely related to human cathepsin L. Using the rat choriocarcinoma cell line model, Rcho-1, mRNA for the PECs cathepsins P, M, Q, R, 1, 2 was found to increase in expression during differentiation into a trophoblast giant cell phenotype. By contrast, expression of cathepsin L was not regulated. A specific enzyme assay was developed to show that activity of cathepsin P mirrored mRNA expression during differentiation. Cathepsin P protein co-localizes with cathepsin B, indicating that the enzyme probably functions in the endosomal-lysosomal compartment. This study demonstrates that the PEC genes produce functional proteases that can perform specific placental roles that are probably performed by broader specificity proteases in human placenta.

Expression of cathepsin P mRNA, protein and activity in the rat choriocarcinoma cell line, Rcho-1, during giant cell transformation. Publishing Authors By Initials

M Hassanein,BD Korant,G Lu,RW Mason,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE: 2007 Aug-Sep

Expression of cathepsin P mRNA, protein and activity in the rat choriocarcinoma cell line, Rcho-1, during giant cell transformation. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Placenta

VOLUME: 28

Page Numbers: 912-9

Journal Abbreviation: Placenta

ISSN: 0143-4004

DAY: 10

MONTH: 01

YEAR: 2007

Expression of cathepsin P mRNA, protein and activity in the rat choriocarcinoma cell line, Rcho-1, during giant cell transformation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8006349

Expression of cathepsin P mRNA, protein and activity in the rat choriocarcinoma cell line, Rcho-1, during giant cell transformation. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Expression of cathepsin P mRNA, protein and activity in the rat choriocarcinoma cell line, Rcho-1, during giant cell transformation. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Expression of cathepsin P mRNA, protein and activity in the rat choriocarcinoma cell line, Rcho-1, during giant cell transformation.

AFFILIATION: Department of Biomedical Research, Alfred I duPont Hospital for Children, 1600 Rock land Road, Wilmington, DE 19803, USA.

Country: England

AGENCY: United States NCRR

GRANT: P20RR20173

ACRONYM: RR

MEDLINETA: Placenta

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Expression of cathepsin P mRNA, protein and activity in the rat choriocarcinoma cell line, Rcho-1, during giant cell transformation Related Publications

• 'Tissue surrogates' as a model for archival formalin-fixed paraffin-embedded tissues.
• 19F-NMR detection of lacZ gene expression via the enzymic hydrolysis of 2-fluoro-4-nitrophenyl beta-D-galactopyranoside in vivo in PC3 prostate tumor xenografts in the mouse.
• A four base pair insertion in exon 1 of the RPS19 gene is a common polymorphism in African-Americans.
• A novel murine protein with no effect on iron homoeostasis is homologous with transferrin and is the putative inhibitor of carbonic anhydrase.
• Cells depleted for RPS19, a protein associated with Diamond Blackfan Anemia, show defects in 18S ribosomal RNA synthesis and small ribosomal subunit production.
• Construction of an infectious cDNA clone for a Brazilian prototype strain of dengue virus type 1: characterization of a temperature-sensitive mutation in NS1.
• Correlation of radiation response with tumor oxygenation in the Dunning prostate R3327-AT1 tumor.
• Differentiated human alveolar epithelial cells and reversibility of their phenotype in vitro.
• Disclosure of positive HIV serostatus by men who have sex with men to family and friends over time.
• Dysfunctional telomeres and dyskeratosis congenita.
• Expression of cathepsin P mRNA, protein and activity in the rat choriocarcinoma cell line, Rcho-1, during giant cell transformation.
• Folate depletion in human lymphocytes up-regulates p53 expression despite marked induction of strand breaks in exons 5-8 of the gene.
• Gene-enhanced tissue engineering for dental hard tissue regeneration: (1) overview and practical considerations.
• Gene-enhanced tissue engineering for dental hard tissue regeneration: (2) dentin-pulp and periodontal regeneration.
• Genetic transformation of tobacco NT1 cells with Agrobacterium tumefaciens.
• Glutathione-induced radical formation on lactoperoxidase does not correlate with the enzyme's peroxidase activity.
• Immuno-spin trapping analyses of DNA radicals.
• International community consensus standard for reporting derivation of human embryonic stem cell lines.
• Mutations in West Nile virus nonstructural proteins that facilitate replicon persistence in vitro attenuate virus replication in vitro and in vivo.
• Preliminary evidence of reduced occipital GABA concentrations in puerperal women: a 1H-MRS study.
• Pressure ulcers and their treatment and effects on quality of life: hospital inpatient perspectives.
• Raphe magnus neurons help protect reactions to visceral pain from interruption by cutaneous pain.
• Reverse transcriptase activity in patients with primary biliary cirrhosis and other autoimmune liver disorders.
• Sonochemistry and the environment - Providing a "green" link between chemistry, physics and engineering.
• Spontaneous haemoperitoneum caused by inferior pancreaticoduodenal artery aneurysm rupture: case report.
• Statistical-mechanical theory of passive transport through partially sieving or leaky membranes.
• The impact of dose escalated radiotherapy for prostate cancer.
• The liver of woodchucks chronically infected with the woodchuck hepatitis virus contains foci of virus core antigen-negative hepatocytes with both altered and normal morphology.
• Transcriptional activity of the telomeric retrotransposon HeT-A in Drosophila melanogaster is stimulated as a consequence of subterminal deficiencies at homologous and nonhomologous telomeres.
• Ultra-high resolution imaging by fluorescence photoactivation localization microscopy.