Expression of bone extracellular matrix proteins on osteoblast cells in the presence of mineral trioxide.

Expression of bone extracellular matrix proteins on osteoblast cells in the presence of mineral trioxide. Research Abstract Details 

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Expression of bone extracellular matrix proteins on osteoblast cells in the presence of mineral trioxide. Abstract Text:

Nobuyuki Tani-Ishii,Nobushiro Hamada,Kiyoko Watanabe,Yasuhisa Tujimoto,Toshio Teranaka,Toshio Umemoto,

The biocompatibility of periapical tissue with mineral trioxide aggregate (MTA) affects its ability to repair and regenerate itself. Here we report the cytotoxicity of MTA and how it affects the expression of bone extracellular matrix protein in MC3T3-E1 osteoblast cells. We quantified the cytotoxicity of MTA, amalgam, and Dycal (Dentsply/Caulk, Milford, DE) on MC3T3-E1 cells by measuring the ability of cells to cleave a tetrazolium salt to produce formazan dye during a period of 24, 48, or 96 hours. We used reverse-transcriptase polymerase chain reaction with primer sets for type I collagen, osteocalcin, and bone sialoprotein to measure the gene-expression response of MC3T3-E1 cells treated with MTA. MTA, amalgam, and Dycal were less toxic after 48 hours. MC3T3-E1 cell growth with MTA and Dycal was greater than nonstimulated controls. MTA caused an upregulation of type I collagen and osteocalcin messenger RNA expression after 24 hours. These results showed that, in the presence of MTA, cells grow faster and produce more mineralized matrix gene expression in osteoblasts.

Expression of bone extracellular matrix proteins on osteoblast cells in the presence of mineral trioxide. Publishing Authors By Initials

N Tani-Ishii,N Hamada,K Watanabe,Y Tujimoto,T Teranaka,T Umemoto,

For similar natural sciences: time: time factors research abstracts see: natural sciences: time: time factors research

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Expression of bone extracellular matrix proteins on osteoblast cells in the presence of mineral trioxide. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of endodontics

VOLUME: 33

Page Numbers: 836-9

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ISSN: 0099-2399

DAY: 18

MONTH: 05

YEAR: 2007

Expression of bone extracellular matrix proteins on osteoblast cells in the presence of mineral trioxide. Information

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LANGUAGE: eng

NlmUniqueID: 7511484

Expression of bone extracellular matrix proteins on osteoblast cells in the presence of mineral trioxide. Keywords Mesh Terms:

KEYWORDS: Time Factors

MESH TERMS: toxicity

Chemical & Substance for Abstract: Expression of bone extracellular matrix proteins on osteoblast cells in the presence of mineral trioxide. Information

Substance Name: Dental Amalgam

Registry Number: 8049-85-2

Grant and Affiliation Information for Expression of bone extracellular matrix proteins on osteoblast cells in the presence of mineral trioxide.

AFFILIATION: Kanagawa Dental College, Yokosuka, Kanagawa, Japan.

Country: United States

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MEDLINETA: J Endod

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