Expression of beta3-adrenoceptor and stimulation of glucose transport by beta3-agonists in brown adipocyte primary culture.

Expression of beta3-adrenoceptor and stimulation of glucose transport by beta3-agonists in brown adipocyte primary culture. Research Abstract Details 

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Expression of beta3-adrenoceptor and stimulation of glucose transport by beta3-agonists in brown adipocyte primary culture. Abstract Text:

H Nikami,Y Shimizu,M Sumida,Y Minokoshi,T Yoshida,M Saito,T Shimazu,

Precursor cells of brown adipocytes were isolated from the interscapular brown fat of newborn rats and cultured on collagen-coated dishes. When confluent cells were treated with dexamethasone, mRNAs for muscle/adipocyte type of glucose transporter, hormone-sensitive lipase, and CCAAT/enhancer binding protein alpha were increased remarkably, confirming a predominant effect of dexamethasone on the terminal differentiation of the cultured cells. Effects of dexamethasone on the expression of three subtypes of beta-adrenoceptor were also examined. beta1- and beta2-adrenoceptor mRNAs remained constant regardless of dexamethasone-treatment, while beta3-adrenoceptor mRNA was present only in dexamethasone-treated differentiated cells. To assess the metabolic response mediated by beta3-adrenoceptor, glucose transport into the cells was estimated. Norepinephrine enhanced glucose transport in dexamethasone-treated differentiated cells, but not in undifferentiated cells. beta3-Adrenergic agonists mimicked completely the stimulatory effect of norepinephrine at concentrations lower by two orders of magnitude. These results suggest that the beta3-adrenoceptor is expressed during the course of differentiation in brown adipocytes and plays a significant role in the response of glucose transport to adrenergic stimulation.

Expression of beta3-adrenoceptor and stimulation of glucose transport by beta3-agonists in brown adipocyte primary culture. Publishing Authors By Initials

H Nikami,Y Shimizu,M Sumida,Y Minokoshi,T Yoshida,M Saito,T Shimazu,

For similar proteins: membrane proteins: receptors, cell surface: receptors, biogenic amine: receptors, catecholamine: receptors, adrenergic: receptors, adrenergic, beta research abstracts see: proteins: membrane proteins: receptors, cell surface: receptors, biogenic amine: receptors, catecholamine: receptors, adrenergic: receptors, adrenergic, beta research

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Expression of beta3-adrenoceptor and stimulation of glucose transport by beta3-agonists in brown adipocyte primary culture. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 119

Page Numbers: 120-5

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jan

YEAR: 1996

Expression of beta3-adrenoceptor and stimulation of glucose transport by beta3-agonists in brown adipocyte primary culture. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Expression of beta3-adrenoceptor and stimulation of glucose transport by beta3-agonists in brown adipocyte primary culture. Keywords Mesh Terms:

KEYWORDS: Receptors, Adrenergic, beta

MESH TERMS: biosynthesis

Chemical & Substance for Abstract: Expression of beta3-adrenoceptor and stimulation of glucose transport by beta3-agonists in brown adipocyte primary culture. Information

Substance Name: Phospholipases A

Registry Number: EC 3.1.1.-

Grant and Affiliation Information for Expression of beta3-adrenoceptor and stimulation of glucose transport by beta3-agonists in brown adipocyte primary culture.

AFFILIATION: Laboratory of Biochemistry, Graduate School of Veterinary Medicine, Hokkaido University, Kita-ku, Sapporo.

Country: JAPAN

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MEDLINETA: J Biochem

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