[Expression of amino terminal of nucleoprotein and glycoprotein G2 of Hantaan virus in insect cells in the form of fusion protein]

[Expression of amino terminal of nucleoprotein and glycoprotein G2 of Hantaan virus in insect cells in the form of fusion protein] Research Abstract Details 

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[Expression of amino terminal of nucleoprotein and glycoprotein G2 of Hantaan virus in insect cells in the form of fusion protein] Abstract Text:

Yong Liu,Zhi-Kai Xu,Fang-Lin Zhang,Wen Luo,Xing-An Wu,Wen-Tao Bai,Gang Hu,

AIM: To express the glycoprotein G2 and amino terminal of nucleoprotein (NP) of Hantaan virus in Bac-to-Bac baculovirus expression system in the form of fusion protein. METHODS: The recombinant baculovirus expression vector pFBDHTa-G2S 0.7 was constructed. The chimeric gene was inserted into bacmid in E.coli DH10Bac with the help of Tn7 transposition system. Then the recombinant baculovirus was screened and the fusion protein was expressed in insect cells. The expression product was detected by ELISA, immunofluorescence assay and Western blot analysis. RESULTS: The recombinant baculovirus containing the chimeric gene G2S 0.7 had been constructed successfully and the fusion protein could be expressed in insect cells. The expressed protein could be recognized by the Hantaan virus NP-specific mAb and glycoprotein G2-specific mAb. CONCLUSION: The successful expression of fusion protein G2S 0.7 with biological activity in insect cells lays the foundation for further research on its immunological characteristic.

[Expression of amino terminal of nucleoprotein and glycoprotein G2 of Hantaan virus in insect cells in the form of fusion protein] Publishing Authors By Initials

Y Liu,ZK Xu,FL Zhang,W Luo,XA Wu,WT Bai,G Hu,

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[Expression of amino terminal of nucleoprotein and glycoprotein G2 of Hantaan virus in insect cells in the form of fusion protein] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese jour

VOLUME: 19

Page Numbers: 215-7

Journal Abbreviation: Xi Bao Yu Fen Zi Mian Yi Xue Z

ISSN: 1007-8738

DAY: 24

MONTH: May

YEAR: 2003

[Expression of amino terminal of nucleoprotein and glycoprotein G2 of Hantaan virus in insect cells in the form of fusion protein] Information

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LANGUAGE: chi

NlmUniqueID: 101139110

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Grant and Affiliation Information for [Expression of amino terminal of nucleoprotein and glycoprotein G2 of Hantaan virus in insect cells in the form of fusion protein]

AFFILIATION: Department of Microbiology, Fourth Military Medical University, Xi'an 710032, China.

Country: China

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MEDLINETA: Xi Bao Yu Fen Zi Mian Yi Xue Z

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