Expression of Aedes trypsin-modulating oostatic factor on the virion of TMV: A potential larvicide.

Expression of Aedes trypsin-modulating oostatic factor on the virion of TMV: A potential larvicide. Research Abstract Details 

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Expression of Aedes trypsin-modulating oostatic factor on the virion of TMV: A potential larvicide. Abstract Text:

Dov Borovsky,Shailaja Rabindran,William O Dawson,Charles A Powell,Donna A Iannotti,Timothy J Morris,Jeffry Shabanowitz,Donald F Hunt,Hendrik L DeBondt,Arnold DeLoof,

We report the engineering of the surface of the tobacco mosaic virus (TMV) virion with a mosquito decapeptide hormone, trypsin-modulating oostatic factor (TMOF). The TMV coat protein (CP) was fused to TMOF at the C terminus by using a read-through, leaky stop codon that facilitated expression of CP and chimeric CP-TMOF (20:1 ratio) that were coassembled into virus particles in infected Nicotiana tabacum. Plants that were infected with the hybrid TMV RNA accumulated TMOF to levels of 1.3% of total soluble protein. Infected tobacco leaf discs that were fed to Heliothis virescens fourth-instar larvae stunted their growth and inhibited trypsin and chymotrypsin activity in their midgut. Purified CP-TMOF virions fed to mosquito larvae stopped larval growth and caused death. Because TMV has a wide host range, expressing TMV-TMOF in plants can be used as a general method to protect them against agricultural insect pests and to control vector mosquitoes.

Expression of Aedes trypsin-modulating oostatic factor on the virion of TMV: A potential larvicide. Publishing Authors By Initials

D Borovsky,S Rabindran,WO Dawson,CA Powell,DA Iannotti,TJ Morris,J Shabanowitz,DF Hunt,HL DeBondt,A DeLoof,

For similar viruses: virion research abstracts see: viruses: virion research

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Expression of Aedes trypsin-modulating oostatic factor on the virion of TMV: A potential larvicide. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 103

Page Numbers: 18963-8

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 0027-8424

DAY: 5

MONTH: 12

YEAR: 2006

Expression of Aedes trypsin-modulating oostatic factor on the virion of TMV: A potential larvicide. Information

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LANGUAGE: eng

NlmUniqueID: 7505876

Expression of Aedes trypsin-modulating oostatic factor on the virion of TMV: A potential larvicide. Keywords Mesh Terms:

KEYWORDS: Virion

MESH TERMS: metabolism

Chemical & Substance for Abstract: Expression of Aedes trypsin-modulating oostatic factor on the virion of TMV: A potential larvicide. Information

Substance Name: Trypsin

Registry Number: EC 3.4.21.4

Grant and Affiliation Information for Expression of Aedes trypsin-modulating oostatic factor on the virion of TMV: A potential larvicide.

AFFILIATION: Florida Medical Entomology Laboratory, University of Florida-Institute of Food and Agricultural Sciences (IFAS), 200 Ninth Street Southeast, Vero Beach, FL 32962-4699, USA. dobo@mail.ifas.ufl.edu

Country: United States

AGENCY: United States NIGMS

GRANT: GM 37537

ACRONYM: GM

MEDLINETA: Proc Natl Acad Sci U S A

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