Expression of active hBMP2 in transgenic tobacco plants.

Expression of active hBMP2 in transgenic tobacco plants. Research Abstract Details 

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Expression of active hBMP2 in transgenic tobacco plants. Abstract Text:

Guangli Suo,Bing Chen,Jingyu Zhang,Yuan Gao,Xia Wang,Zhengquan He,Jianwu Dai,

Bone morphogenetic protein 2 (BMP2) is important for bone tissue repair. The goal of this research is to construct a high level human BMP2 (hBMP2) expression system using transgenic tobacco plants as a bioreactor. Cauliflower mosaic virus (CaMV) 35S promoter, alfalfa mosaic virus (AMV) enhancer, tobacco mosaic virus (TMV) enhancer, matrix attachment regions (MARs) sequence, and "Kozak" sequence were used to construct recombinant expression vectors and the high-expression vectors were screened out through GUS-fusions assay. The promoter is the most important factor; double-CaMV 35S promoter is more effective than single promoter. The AMV or TMV enhancer is able to promote the foreign protein expression. After four-step purification, the activated hBMP2 (0.02% total soluble protein) was obtained. Our results suggested that the transgenic tobacco has great potential to be used as a bioreactor to produce hBMP2.

Expression of active hBMP2 in transgenic tobacco plants. Publishing Authors By Initials

G Suo,B Chen,J Zhang,Y Gao,X Wang,Z He,J Dai,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

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Expression of active hBMP2 in transgenic tobacco plants. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Plant cell reports

VOLUME: 25

Page Numbers: 1316-24

Journal Abbreviation: Plant Cell Rep.

ISSN: 0721-7714

DAY: 4

MONTH: 07

YEAR: 2006

Expression of active hBMP2 in transgenic tobacco plants. Information

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LANGUAGE: eng

NlmUniqueID: 9880970

Expression of active hBMP2 in transgenic tobacco plants. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: metabolism

Chemical & Substance for Abstract: Expression of active hBMP2 in transgenic tobacco plants. Information

Substance Name: Glucuronidase

Registry Number: EC 3.2.1.31

Grant and Affiliation Information for Expression of active hBMP2 in transgenic tobacco plants.

AFFILIATION: Center of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100080, China.

Country: Germany

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MEDLINETA: Plant Cell Rep

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