Expression of a ribosome-inactivating protein gene in bitter melon is induced by Sphaerotheca fuliginea and abiotic stimuli.

Expression of a ribosome-inactivating protein gene in bitter melon is induced by Sphaerotheca fuliginea and abiotic stimuli. Research Abstract Details 

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Expression of a ribosome-inactivating protein gene in bitter melon is induced by Sphaerotheca fuliginea and abiotic stimuli. Abstract Text:

Jun Xu,Hualin Wang,Jianming Fan,Jun Xu,Hualin Wang,Jianming Fan,Jun Xu,Hualin Wang,Jianming Fan,

The gene encoding a single-chain, ribosome-inactivating protein (SCRIP) was cloned from bitter melon (Momordica charantia L.) leaves infected with the fungus, Sphaerotheca fuliginea, by RT-PCR. The ORF was 861 bp. The ribosome-inactivating protein was expressed in E. coli and, when purified, it inhibited the growth of the Sphaerotheca fuliginea in vitro. Northern blot analysis revealed that RIP transcripts rapidly accumulated in leaves 1-day post inoculation with Sphaerotheca fuliginea and reached a peak at 3 d. The expression pattern of RIP induced by methyl jasmonate and salicylic acid were different from that of pathogen-induced expression. Mechanical wounding, silver nitrate and osmotic stress stimulated only a slight accumulation of RIP transcripts. Abscisic acid also induced transcription of RIPs. The signal compounds, ethylene and okadaic acid, induced a moderate accumulation of RIP transcripts.

Expression of a ribosome-inactivating protein gene in bitter melon is induced by Sphaerotheca fuliginea and abiotic stimuli. Publishing Authors By Initials

J Xu,H Wang,J Fan,J Xu,H Wang,J Fan,J Xu,H Wang,J Fan,

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Expression of a ribosome-inactivating protein gene in bitter melon is induced by Sphaerotheca fuliginea and abiotic stimuli. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biotechnology letters

VOLUME: 29

Page Numbers: 1605-10

Journal Abbreviation: Biotechnol. Lett.

ISSN: 0141-5492

DAY: 20

MONTH: 06

YEAR: 2007

Expression of a ribosome-inactivating protein gene in bitter melon is induced by Sphaerotheca fuliginea and abiotic stimuli. Information

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LANGUAGE: eng

NlmUniqueID: 8008051

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Grant and Affiliation Information for Expression of a ribosome-inactivating protein gene in bitter melon is induced by Sphaerotheca fuliginea and abiotic stimuli.

AFFILIATION: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.

Country: Netherlands

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MEDLINETA: Biotechnol Lett

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