Expression of 5-lipoxygenase in specialized epithelial cells of nasopharyngeal-associated lymphoid tissue.

Expression of 5-lipoxygenase in specialized epithelial cells of nasopharyngeal-associated lymphoid tissue. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Expression of 5-lipoxygenase in specialized epithelial cells of nasopharyngeal-associated lymphoid tissue. Abstract Text:

Thomas G Brock,

Leukotrienes are lipid mediators that are produced primarily by certain types of leukocytes. The synthesis of the leukotriene LTB(4) is initiated by the enzyme 5-lipoxygenase and completed by LTA(4) hydrolase. Epithelial cells constitutively express LTA(4) hydrolase but normally lack 5-lipoxygenase. In this study, we report that the stratified squamous epithelial cells from inflamed or hyperplastic tissues of palatine and pharyngeal tonsils (nasopharyngeal-associated lymphoid tissue) express 5-lipoxygenase protein. The localization of 5-lipoxygenase was indicated by immunohistochemical staining and presence confirmed by immunoblot. Positive staining for 5-lipoxygenase in infiltrating leukocytes in inflamed tissues served as internal positive controls for immunohistochemical staining. Staining for 5-lipoxygenase in appendix tissue was negative for epithelial cells while positive for polymorphonuclear leukocytes, indicating that 5-lipoxygenase expression is not a general feature of epithelial cells in mucosa-associated lymphoid tissue. In tonsils, 5-lipoxygenase staining was pronounced in broad regions but reduced or absent in others, suggesting regional regulation of expression. Epithelial cells of tonsils were also positive for 5-lipoxygenase activating protein and leukotriene A(4) hydrolase, indicating a capacity to produce LTB(4). Taken together, these results suggest that the specialized epithelial cells of the mucosa-associated lymphoid tissue of human tonsils can synthesize LTB(4). This lipid mediator may serve to modulate the function of cells within the lymphoid tissue as well as promote an inflammatory response.

Expression of 5-lipoxygenase in specialized epithelial cells of nasopharyngeal-associated lymphoid tissue. Publishing Authors By Initials

TG Brock,

For similar oropharynx: tonsil research abstracts see: oropharynx: tonsil research

PUBMED ID PMID:

MEDLINE DATE:

Expression of 5-lipoxygenase in specialized epithelial cells of nasopharyngeal-associated lymphoid tissue. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of molecular histology

VOLUME: 36

Page Numbers: 475-81

Journal Abbreviation: J. Mol. Histol.

ISSN: 1567-2379

DAY: 28

MONTH: 04

YEAR: 2006

Expression of 5-lipoxygenase in specialized epithelial cells of nasopharyngeal-associated lymphoid tissue. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101193653

Expression of 5-lipoxygenase in specialized epithelial cells of nasopharyngeal-associated lymphoid tissue. Keywords Mesh Terms:

KEYWORDS: Tonsil

MESH TERMS: enzymology

Chemical & Substance for Abstract: Expression of 5-lipoxygenase in specialized epithelial cells of nasopharyngeal-associated lymphoid tissue. Information

Substance Name: Arachidonate 5-Lipoxygenase

Registry Number: EC 1.13.11.34

Grant and Affiliation Information for Expression of 5-lipoxygenase in specialized epithelial cells of nasopharyngeal-associated lymphoid tissue.

AFFILIATION: Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109-0642, USA. brocko@umich.edu

Country: Netherlands

AGENCY: United States NIAID

GRANT: R01 AI43574

ACRONYM: AI

MEDLINETA: J Mol Histol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Expression of 5-lipoxygenase in specialized epithelial cells of nasopharyngeal-associated lymphoid tissue Related Publications

• A case series of HIV-positive patients with malignant melanoma.
• A general methodology for the synthesis of transition metal pnictide nanoparticles from pnictate precursors and its application to iron-phosphorus phases.
• An HIV-associated mediastinal germ cell tumour complicated by acute myeloid leukaemia.
• An artificial mitochondrial tail signal/anchor sequence confirms a requirement for moderate hydrophobicity for targeting.
• An improved system for the generation and analysis of mutant proteins containing unnatural amino acids in Saccharomyces cerevisiae.
• An unexpected co-crystal with a variable degree of order: 1:1 rac-1,2-cyclohexanediol/triphenylphosphine oxide.
• Congenital cyanotic heart disease.
• Contact surface and material nonlinearity modeling of human lungs.
• Daily administration of phosphodiesterase type 5 inhibitors for urological and nonurological indications.
• Drainage, streptomycin and tuberculosis.
• Evaluation of hunter-harvested white-tailed deer for evidence of bovine viral diarrhea virus infection in Alabama.
• Expression of 5-lipoxygenase in specialized epithelial cells of nasopharyngeal-associated lymphoid tissue.
• First stage approximation of the exstrophic bladder in patients with cloacal exstrophy--should this be the initial surgical approach in all patients?
• HOW THE CONFEDERATE ARMY WAS VACCINATED.
• Leukotrienes: mediators that have been typecast as villains.
• Malnutrition in South Africa.
• Morbus coeruleus; a study of 50 cases after the Blalock-Taussig operation.
• Particle-rod hybrids: growth of arachidic acid molecular rods from capped cadmium selenide nanoparticles.
• Potential mRNA degradation targets of hsa-miR-200c, identified using informatics and qRT-PCR.
• Surgical outcomes and patient satisfaction after dermal, pericardial, and small intestinal submucosal grafting for Peyronie's disease.
• Synthesis of MnP nanocrystals by treatment of metal carbonyl complexes with phosphines: a new, versatile route to nanoscale transition metal phosphides.
• The activity of lipopeptide TLR2 agonists critically depends on the presence of solubilizers.
• The impact of a fellowship on resident training in an academic pediatric urology practice.
• The role of patients' expectations and goals in the behavioral and pharmacological treatment of obesity.
• The surgery of pulmonary stenosis.
• The surgery of the heart.
• Transcriptional interference: an unexpected layer of complexity in gene regulation.
• [Not Available.]
• [Not Available.]
• miRNA: the new gene silencer.