Expression in Escherichia coli, purification and characterization of Thermoanaerobacter tengcongensis ribosome recycling factor.

Expression in Escherichia coli, purification and characterization of Thermoanaerobacter tengcongensis ribosome recycling factor. Research Abstract Details 

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Expression in Escherichia coli, purification and characterization of Thermoanaerobacter tengcongensis ribosome recycling factor. Abstract Text:

Peng Guo,Liqiang Zhang,Zhen Qi,Runsheng Chen,Guozhong Jing,

A very promising approach to understanding the mechanism of protein thermostability is to investigate the structure-function relationship of homologous proteins with different thermostabilities. Ribosome recycling factor (RRF), which is an essential factor for protein synthesis in bacteria, may be a good candidate for such study. In this report, a ribosome recycling factor from Thermoanaerobacter tengcongensis was expressed and characterized. This protein contains 184 residues, shows 51.4% identity to that of Escherichia coli RRF, and has very strong antigenic cross-reactivity with antibody to E. coli RRF. In vivo activity assay shows that weak residual activity may remain in TteRRF in E. coli cells. Circular dichroism spectral analysis shows that TteRRF has a very similar secondary structure to that of E. coli RRF, implying that they have similar tertiary structures. However, their thermostabilities are significantly different. To find which domain of RRF is mainly responsible for maintaining stability, TteDI/EcoDII and EcoDI/TteDII RRF chimeras were created. Their domain I and domain II are from E. coli and T. tengcongensis RRFs, respectively. The results of GdnHCl and heat induced denaturation of the chimeric RRFs suggest that the domain I plays a major role in maintaining the stability of the RRF molecule.

Expression in Escherichia coli, purification and characterization of Thermoanaerobacter tengcongensis ribosome recycling factor. Publishing Authors By Initials

P Guo,L Zhang,Z Qi,R Chen,G Jing,

For similar bacteria: gram-positive bacteria: gram-positive rods: gram-positive asporogenous rods: gram-positive asporogenous rods, irregular: thermoanaerobacter research abstracts see: bacteria: gram-positive bacteria: gram-positive rods: gram-positive asporogenous rods: gram-positive asporogenous rods, irregular: thermoanaerobacter research

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Expression in Escherichia coli, purification and characterization of Thermoanaerobacter tengcongensis ribosome recycling factor. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 138

Page Numbers: 89-94

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jul

YEAR: 2005

Expression in Escherichia coli, purification and characterization of Thermoanaerobacter tengcongensis ribosome recycling factor. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Expression in Escherichia coli, purification and characterization of Thermoanaerobacter tengcongensis ribosome recycling factor. Keywords Mesh Terms:

KEYWORDS: Thermoanaerobacter

MESH TERMS: metabolism

Chemical & Substance for Abstract: Expression in Escherichia coli, purification and characterization of Thermoanaerobacter tengcongensis ribosome recycling factor. Information

Substance Name: ribosome releasing factor

Registry Number: 0

Grant and Affiliation Information for Expression in Escherichia coli, purification and characterization of Thermoanaerobacter tengcongensis ribosome recycling factor.

AFFILIATION: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, PR China.

Country: Japan

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MEDLINETA: J Biochem

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