Expression and release of the a and b subunits for human coagulation factor XIII in baby hamster kidney (BHK) cells.

Expression and release of the a and b subunits for human coagulation factor XIII in baby hamster kidney (BHK) cells. Research Abstract Details 

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Expression and release of the a and b subunits for human coagulation factor XIII in baby hamster kidney (BHK) cells. Abstract Text:

H Kaetsu,T Hashiguchi,D Foster,A Ichinose,

The a subunit of coagulation factor XIII lacks a hydrophobic signal sequence for secretion from cells, while the b subunit has a typical signal sequence. To determine whether the a subunit can be synthesized and released, expression vectors containing the cDNA for either subunit were transfected into baby hamster kidney (BHK) cells. Western blotting analysis and gel filtration chromatography demonstrated that the recombinant a and b subunits (rXIIIa and rXIIIb) had the same molecular weights and subunit structures (a2, b2, and a2b2) as the native molecules. rXIIIa was enzymatically active when activated by thrombin. Most rXIIIb was secreted as measured by ELISA, while most rXIIIa was detected in the cytosol by subcellular fractionation. Co-expression with rXIIIb in the same cells did not promote the release of rXIIIa. Treatment of the cells with brefeldin A, a potent inhibitor of protein transportation, blocked the secretion of rXIIIb, although it had no effect on the release of rXIIIa. Several drugs and heat stress induced the release of rXIIIa, which correlated directly with that of cytoplasmic lactate dehydrogenase. These results suggest that the a subunit is released from cells as a consequence of cell injury, which is independent of the classical secretory pathway.

Expression and release of the a and b subunits for human coagulation factor XIII in baby hamster kidney (BHK) cells. Publishing Authors By Initials

H Kaetsu,T Hashiguchi,D Foster,A Ichinose,

For similar investigative techniques: genetic techniques: gene transfer techniques: transfection research abstracts see: investigative techniques: genetic techniques: gene transfer techniques: transfection research

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Expression and release of the a and b subunits for human coagulation factor XIII in baby hamster kidney (BHK) cells. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of biochemistry

VOLUME: 119

Page Numbers: 961-9

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: May

YEAR: 1996

Expression and release of the a and b subunits for human coagulation factor XIII in baby hamster kidney (BHK) cells. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Expression and release of the a and b subunits for human coagulation factor XIII in baby hamster kidney (BHK) cells. Keywords Mesh Terms:

KEYWORDS: Transfection

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Expression and release of the a and b subunits for human coagulation factor XIII in baby hamster kidney (BHK) cells. Information

Substance Name: Thrombin

Registry Number: EC 3.4.21.5

Grant and Affiliation Information for Expression and release of the a and b subunits for human coagulation factor XIII in baby hamster kidney (BHK) cells.

AFFILIATION: Department of Biochemistry, University of Washington, Seattle 98195, USA.

Country: JAPAN

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MEDLINETA: J Biochem

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