Expression and inheritance of hypersensitive resistance to rice hoja blanca virus mediated by the viral nucleocapsid protein gene in transgenic rice.

Expression and inheritance of hypersensitive resistance to rice hoja blanca virus mediated by the viral nucleocapsid protein gene in transgenic rice. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Expression and inheritance of hypersensitive resistance to rice hoja blanca virus mediated by the viral nucleocapsid protein gene in transgenic rice. Abstract Text:

Z Lentini,I Lozano,E Tabares,L Fory,J Domínguez,M Cuervo,L Calvert,

Rice hoja blanca virus (RHBV) is a major virus disease of economic importance affecting rice in northern South America, Central America and the Caribbean. This is the first report of transgenic resistance to RHBV and the transformation of an indica rice variety from Latin America. Rice transformed with the RHBV nucleocapsid protein ( N) gene had a significant reduction in disease development. Several reactions were observed that ranged from susceptible to completely resistant plants (immunity). The resistant reactions were characterized by the production of local lesions like a hypersensitive reaction or a recovery phenotype with the emergence of symptom-less new leaves. These transgenic RHBV-resistant rice lines expressed the N gene RNA at low levels that were below the detection limit by Northern blots and only resolved by RT-PCR. The nucleocapsid protein could not be detected in any of the transgenic plants either by Western or ELISA tests. These results suggest that the resistance encoded by the N gene in these plants appears to be mediated by RNA. When challenged with RHBV, the resistant transgenic lines showed a significant increased performance for important agronomic traits including the number of tillers, the number of grains per plant and the yield as compared to the susceptible control. Furthermore, upon inoculation some of the most-resistant transgenic lines showed agronomic traits similar to the uninoculated non-transgenic Cica 8 control. Using both agronomic traits and disease severity as criteria, several of the most-resistant lines were followed through the R(4) generation and demonstrated that the N gene and RHBV resistance was inherited in a stable manner. These transgenic rice lines could become a new genetic resource in developing RHBV-resistant cultivars.

Expression and inheritance of hypersensitive resistance to rice hoja blanca virus mediated by the viral nucleocapsid protein gene in transgenic rice. Publishing Authors By Initials

Z Lentini,I Lozano,E Tabares,L Fory,J Domínguez,M Cuervo,L Calvert,

For similar viruses: plant viruses research abstracts see: viruses: plant viruses research

PUBMED ID PMID:

MEDLINE DATE:

Expression and inheritance of hypersensitive resistance to rice hoja blanca virus mediated by the viral nucleocapsid protein gene in transgenic rice. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: TAG. Theoretical and applied genetics. Theoretisch

VOLUME: 106

Page Numbers: 1018-26

Journal Abbreviation:

ISSN: 0040-5752

DAY: 14

MONTH: 12

YEAR: 2002

Expression and inheritance of hypersensitive resistance to rice hoja blanca virus mediated by the viral nucleocapsid protein gene in transgenic rice. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 145600

Expression and inheritance of hypersensitive resistance to rice hoja blanca virus mediated by the viral nucleocapsid protein gene in transgenic rice. Keywords Mesh Terms:

KEYWORDS: Plant Viruses

MESH TERMS: metabolism

Chemical & Substance for Abstract: Expression and inheritance of hypersensitive resistance to rice hoja blanca virus mediated by the viral nucleocapsid protein gene in transgenic rice. Information

Substance Name: Capsid Proteins

Registry Number: 0

Grant and Affiliation Information for Expression and inheritance of hypersensitive resistance to rice hoja blanca virus mediated by the viral nucleocapsid protein gene in transgenic rice.

AFFILIATION: International Center for Tropical Agriculture (CIAT), A.A. 6713, Cali, Colombia. z.lentini@cgiar.org

Country: Germany

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Theor Appl Genet

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Expression and inheritance of hypersensitive resistance to rice hoja blanca virus mediated by the viral nucleocapsid protein gene in transgenic rice Related Publications

• Acute pericarditis.
• Alopecia and cytotoxic drugs.
• Avian IgY Binds to a Monocyte Receptor with IgG-like Kinetics Despite an IgE-like Structure.
• Dietary restriction reduces the rate of estradiol clearance in sheep (Ovis aries).
• Direct spinal anaesthesia for repair of myelomeningocele.
• Expression and inheritance of hypersensitive resistance to rice hoja blanca virus mediated by the viral nucleocapsid protein gene in transgenic rice.
• Fixation of intertrochanteric fractures of the femur. A randomised prospective comparison of the gamma nail and the dynamic hip screw.
• Food Marketing on Popular Children's Web Sites: A Content Analysis.
• Hypothyroidism and lichen myxoedematosus.
• Influence of combined angiotensin-converting enzyme inhibitors and spironolactone on serum K+, Mg 2+, and Na+ concentrations in small dogs with degenerative mitral valve disease.
• Intense Physical Training Modifies Intrinsic Heart Rate And Cardiac Autonomic Modulation: 1263: Board #26 May 30 11:00 AM - 12:30 PM.
• Intrinsic hazard of breech presentation.
• Invisible genomes: the genomics revolution and patenting practice.
• Knowledge-making distinctions in synthetic biology.
• Molluscum contagiosum.
• More hydatidiform moles?
• Oral histories: bridging misconceptions and reality in brain injury recovery.
• Patient package inserts.
• Performance analysis for a K-winners-take-all analog neural network: basic theory.
• Planning for future performance: implications for long term training.
• Prophylactic high-frequency oscillatory ventilation in preterm infants.
• Psychiatric assessment of the aged: a differential model for diagnosis.
• Simultaneous slipped upper femoral epiphysis in identical twins.
• Stress fracture of the acromion: an unusual mechanism and review of the literature.
• Targeting Poly(ADP-Ribose) Polymerase: A Two-Armed Strategy for Cancer Therapy.
• The Gamma nail--a significant advance or a passing fashion?
• The Sanitary Works of Indianapolis.
• The brain stem damage syndrome: speech and psychological factors.
• The premature breech.
• Well-child care in family practice residency clinics.