Expression and immunogenicity of the V3 loop from the envelope of human immunodeficiency virus type 1 in an attenuated aroA strain of Salmonella typhimurium upon genetic coupling to two Escherichia coli carrier proteins.

Expression and immunogenicity of the V3 loop from the envelope of human immunodeficiency virus type 1 in an attenuated aroA strain of Salmonella typhimurium upon genetic coupling to two Escherichia coli carrier proteins. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Expression and immunogenicity of the V3 loop from the envelope of human immunodeficiency virus type 1 in an attenuated aroA strain of Salmonella typhimurium upon genetic coupling to two Escherichia coli carrier proteins. Abstract Text:

A Charbit,P Martineau,J Ronco,C Leclerc,R Lo-Man,V Michel,D O'Callaghan,M Hofnung,

A peptide comprising residues glu293 to ser334 from the principal neutralization determinant (V3 loop) of the envelope of human immunodeficiency virus type 1 (HIV1 LAVBRU isolate) has been inserted within internal permissive sites of either LamB or MalE, two envelope proteins from Escherichia coli K12. The MalE hybrid protein (MalE133-V3 loop) was stably expressed in the periplasm of Escherichia coli K12, and the V3 loop peptide was detectable on the surface of the native protein by an anti-gp160 monoclonal antibody (mAb 110-A). The disulfide bridge between the two cysteines of the loop was formed. In contrast, genetic coupling to the outer membrane protein LamB did not allow the expression of a stable hybrid protein, and major proteolytic cleavage products of the LamB153-V3 loop were detected by mAb 110-A. The two plasmid-encoded hybrid genes were transferred to an aroA mutant of Salmonella typhimurium. Constitutive expression of the MalE133-V3 loop had no detectable effect on cell growth and on the survival in vivo of the recipient strain. The LamB153-V3 loop was not stably expressed in Salmonella, either in vitro or in vivo. Live recombinant salmonellas expressing MalE-V3 and LamB-V3 loop hybrids were used to immunize mice. The MalE-V3 loop hybrid induced anti-HIV1 envelope antibodies detectable by Western blot and ELISA, while the anti-HIV1 envelope antibodies induced by the LamB-V3 loop hybrid were only detectable by Western blot. In addition, purified MalE-V3 loop hybrid protein was able to stimulate in vitro and induce in vivo a V3 loop-specific T-cell proliferative response.

Expression and immunogenicity of the V3 loop from the envelope of human immunodeficiency virus type 1 in an attenuated aroA strain of Salmonella typhimurium upon genetic coupling to two Escherichia coli carrier proteins. Publishing Authors By Initials

A Charbit,P Martineau,J Ronco,C Leclerc,R Lo-Man,V Michel,D O'Callaghan,M Hofnung,

For similar cells: blood cells: leukocytes: leukocytes, mononuclear: lymphocytes: t-lymphocytes research abstracts see: cells: blood cells: leukocytes: leukocytes, mononuclear: lymphocytes: t-lymphocytes research

PUBMED ID PMID:

MEDLINE DATE:

Expression and immunogenicity of the V3 loop from the envelope of human immunodeficiency virus type 1 in an attenuated aroA strain of Salmonella typhimurium upon genetic coupling to two Escherichia coli carrier proteins. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Vaccine

VOLUME: 11

Page Numbers: 1221-8

Journal Abbreviation: Vaccine

ISSN: 0264-410X

DAY: 27

MONTH: Sep

YEAR: 1993

Expression and immunogenicity of the V3 loop from the envelope of human immunodeficiency virus type 1 in an attenuated aroA strain of Salmonella typhimurium upon genetic coupling to two Escherichia coli carrier proteins. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8406899

Expression and immunogenicity of the V3 loop from the envelope of human immunodeficiency virus type 1 in an attenuated aroA strain of Salmonella typhimurium upon genetic coupling to two Escherichia coli carrier proteins. Keywords Mesh Terms:

KEYWORDS: T-Lymphocytes

MESH TERMS: immunology

Chemical & Substance for Abstract: Expression and immunogenicity of the V3 loop from the envelope of human immunodeficiency virus type 1 in an attenuated aroA strain of Salmonella typhimurium upon genetic coupling to two Escherichia coli carrier proteins. Information

Substance Name: maltose-binding protein

Registry Number: 0

Grant and Affiliation Information for Expression and immunogenicity of the V3 loop from the envelope of human immunodeficiency virus type 1 in an attenuated aroA strain of Salmonella typhimurium upon genetic coupling to two Escherichia coli carrier proteins.

AFFILIATION: Programmation Moleculaire et Toxicologie Génétique (CNRS URA 1444), Institut Pasteur, Paris, France.

Country: ENGLAND

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Vaccine

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Expression and immunogenicity of the V3 loop from the envelope of human immunodeficiency virus type 1 in an attenuated aroA strain of Salmonella typhimurium upon genetic coupling to two Escherichia coli carrier proteins Related Publications

• A genetic system to elicit and monitor antipeptide antibodies without peptide synthesis.
• A system for genetic analysis in gene lamB: first results with lambda-resistant tight mutants.
• An intelligent channel (and more).
• Antibodies against synthetic peptides and the topology of LamB, an outer membrane protein from Escherichia coli K12.
• Bacteriophage lambda receptor site on the Escherichia coli K-12 LamB protein.
• Creation of targets for proteolytic cleavage in the LamB protein of E coli K12 by genetic insertion of foreign sequences: implications for topological studies.
• DNA sequence analysis of the lamB gene from Klebsiella pneumoniae: implications for the topology and the pore functions in maltoporin.
• Expression and immunogenicity of the V3 loop from the envelope of human immunodeficiency virus type 1 in an attenuated aroA strain of Salmonella typhimurium upon genetic coupling to two Escherichia coli carrier proteins.
• Expression of a poliovirus neutralization epitope at the surface of recombinant bacteria: first immunization results.
• Expression of foreign antigenic determinants on bacterial envelope proteins.
• Further sequence analysis of the phage lambda receptor site. Possible implications for the organization of the lamB protein in Escherichia coli K12.
• Gene sequence of the lambda receptor, an outer membrane protein of E. coli K12.
• Genetic study of a membrane protein: DNA sequence alterations due to 17 lamB point mutations affecting adsorption of phage lambda.
• Immunodominance of a recombinant T-cell epitope depends on its molecular environment.
• Immunogenicity and antigenicity of conserved peptides from the envelope of HIV-1 expressed at the surface of recombinant bacteria.
• Immunogenicity of viral B- and T-cell epitopes expressed in recombinant bacterial proteins.
• In situ enzyme immunodetection of surface or intracellular bacterial antigens using nitrocellulose sheets.
• In vivo and in vitro studies of transmembrane beta-strand deletion, insertion or substitution mutants of the Escherichia coli K-12 maltoporin.
• Isolation of different bacteriophages using the LamB protein for adsorption on Escherichia coli K-12.
• Linker mutagenesis in the gene of an outer membrane protein of Escherichia coli, lamB.
• Maltose transport and starch binding in phage-resistant point mutants of maltoporin. Functional and topological implications.
• Mutagenesis by random linker insertion into the lamB gene of Escherichia coli K12.
• Mutations in gene lamB: studies on structure and topology of an E. coli outer membrane protein.
• Negative dominance in gene lamB: random assembly of secreted subunits issued from different polysomes.
• Permissive sites and topology of an outer membrane protein with a reporter epitope.
• Presentation of two epitopes of the preS2 region of hepatitis B virus on live recombinant bacteria.
• The cellular location of a foreign B cell epitope expressed by recombinant bacteria determines its T cell-independent or T cell-dependent characteristics.
• The maltoporin of Salmonella typhimurium: sequence and folding model.
• [DNA sequence encoding the signal peptide of the lambda receptor in E. coli K 12]
• rho Mutations restore lamB expression in E. coli K12 strains with an inactive malB region.