Expression and function of COOH-terminal myosin heavy chain isoforms in mouse smooth muscle.

Expression and function of COOH-terminal myosin heavy chain isoforms in mouse smooth muscle. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Expression and function of COOH-terminal myosin heavy chain isoforms in mouse smooth muscle. Abstract Text:

Anne F Martin,Sunita Bhatti,Gail J Pyne-Geithman,Mariam Farjah,Vlasios Manaves,Lori Walker,Roberta Franks,Arthur R Strauch,Richard J Paul,

Isoforms of the smooth muscle myosin motor, SM1 and SM2, differ in length at the carboxy terminal tail region. Their proportion changes with development, hormonal status and disease, but their function is unknown. We developed mice carrying the myosin heavy chain (MyHC) transgenes SM1, cMyc-tagged SM1, SM2, and V5-tagged SM2, and all transgenes corresponded to the SMa NH(2)-terminal isoform. Transgene expression was targeted to smooth muscle by the smooth muscle alpha-actin promoter. Immunoblot analysis showed substantial expression of the cMyc-tagged SM1 and V5-tagged SM2 MyHC protein in aorta and bladder and transgene mRNA was expressed in mice carrying unlabeled SM1 or SM2 transgenes. Despite significant protein expression of tagged MyHCs we found only small changes in the SM1:SM2 protein ratio. Significant changes in functional phenotype were observed in mice carrying unlabeled SM1 or SM2 transgenes. Force in aorta and bladder was increased (72 +/- 14%, 92 +/- 11%) in SM1 and decreased to 57 +/- 1% and 80 +/- 3% in SM2 transgenic mice. SM1 transgenic bladders had faster (1.8 +/- 0.3 s) and SM2 slower (7.1 +/- 0.5 s) rates of force redevelopment following a rapid step shortening. We hypothesize that small changes in the SM1:SM2 ratio could be amplified if they are associated with changes in thick filament assembly and underlie the altered contractility. These data provide evidence indicating an in vivo function for the COOH-terminal isoforms of smooth muscle myosin and suggest that the SM1:SM2 ratio is tightly regulated in smooth muscle tissues.

Expression and function of COOH-terminal myosin heavy chain isoforms in mouse smooth muscle. Publishing Authors By Initials

AF Martin,S Bhatti,GJ Pyne-Geithman,M Farjah,V Manaves,L Walker,R Franks,AR Strauch,RJ Paul,

For similar urogenital system: urinary tract: urinary bladder research abstracts see: urogenital system: urinary tract: urinary bladder research

PUBMED ID PMID:

MEDLINE DATE:

Expression and function of COOH-terminal myosin heavy chain isoforms in mouse smooth muscle. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: American journal of physiology. Cell physiology

VOLUME: 293

Page Numbers: C238-45

Journal Abbreviation: Am. J. Physiol., Cell Physiol.

ISSN: 0363-6143

DAY: 28

MONTH: 03

YEAR: 2007

Expression and function of COOH-terminal myosin heavy chain isoforms in mouse smooth muscle. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100901225

Expression and function of COOH-terminal myosin heavy chain isoforms in mouse smooth muscle. Keywords Mesh Terms:

KEYWORDS: Urinary Bladder

MESH TERMS: metabolism

Chemical & Substance for Abstract: Expression and function of COOH-terminal myosin heavy chain isoforms in mouse smooth muscle. Information

Substance Name: Smooth Muscle Myosins

Registry Number: EC 3.6.1.-

Grant and Affiliation Information for Expression and function of COOH-terminal myosin heavy chain isoforms in mouse smooth muscle.

AFFILIATION: Dept. of Physiology and Biophysics, University of Illinois at Chicago, 835 S. Wolcott Ave., Chicago, IL 60612, USA. afmartin@uic.edu

Country: United States

AGENCY: United States NHLBI

GRANT: HL-64942

ACRONYM: HL

MEDLINETA: Am J Physiol Cell Physiol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Expression and function of COOH-terminal myosin heavy chain isoforms in mouse smooth muscle Related Publications

• Acute cutaneous T-cell lymphoma transformation during treatment with alemtuzumab.
• An exploration into study design for biomarker identification: issues and recommendations.
• Antibodies to the superantigenic site of HIV-1 gp120: hydrolytic and binding activities of the light chain subunit.
• Biventricular non-compaction and giant left atrial appendage.
• Chemical deterrence of a cyanobacterial metabolite against generalized and specialized grazers.
• Child assessment of dextromethorphan, diphenhydramine, and placebo for nocturnal cough due to upper respiratory infection.
• Clinical-radiological features of fractures in premature infants--a review.
• Contact dermatitis to hydrolyzed wheat protein.
• Distinct roles of PMCA isoforms in Ca2+ homeostasis of bladder smooth muscle: evidence from PMCA gene-ablated mice.
• Ecological impact of solar ultraviolet-B (UV-B: 320-290 nm) radiation on Corynebacterium aquaticum and Xanthomonas sp. colonization on tea phyllosphere in relation to blister blight disease incidence in the field.
• Efficacy and safety of aminoglycoside monotherapy: systematic review and meta-analysis of randomized controlled trials.
• Evidence that scabies mites (Acari: Sarcoptidae) influence production of interleukin-10 and the function of T-regulatory cells (Tr1) in humans.
• Evidence that the deficit in sexual behavior in adult rats neonatally exposed to citalopram is a consequence of 5-HT1 receptor stimulation during development.
• Expression and function of COOH-terminal myosin heavy chain isoforms in mouse smooth muscle.
• Genetic and physiological evidence that oligodendrocyte gap junctions contribute to spatial buffering of potassium released during neuronal activity.
• Higher plasma but not intracellular concentrations after infusion with liposomal daunorubicin compared with conventional daunorubicin in adult acute myeloid leukemia.
• How do the vaccine polio viruses replace the wild polio viruses?
• Identifying cardinal dates in phytoplankton time series to enable the analysis of long-term trends.
• Lymphopenic mice reconstituted with limited repertoire T cells develop severe, multiorgan, Th2-associated inflammatory disease.
• Modelling of biological nitrogen removal during treatment of piggery wastewater.
• Modelling of biological processes during aerobic treatment of piggery wastewater aiming at process optimisation.
• Naphthaquinones of Diospyros batocana.
• New insights in chronic allograft rejection.
• SSPE and measles vaccine.
• Smart brackets for 3D-force-moment measurements in orthodontic research and therapy - developmental status and prospects.
• The effect of micro-CN linkage isomerism and ancillary ligand set on directional metal-metal charge-transfer in cyanide-bridged dimanganese complexes.
• The use of atrial overdrive and ventricular rate stabilization pacing algorithms for the prevention and treatment of paroxysmal atrial fibrillation: the Pacemaker Atrial Fibrillation Suppression (PAFS) study.
• Towards irreversible HIV inactivation: stable gp120 binding by nucleophilic antibodies.
• [In Process Citation]
• [In Process Citation]