[Expression activity of maize Ubi-1 promoter in fertile transgenic maize plants]

[Expression activity of maize Ubi-1 promoter in fertile transgenic maize plants] Research Abstract Details 

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[Expression activity of maize Ubi-1 promoter in fertile transgenic maize plants] Abstract Text:

Zi Qin Xu,Jing Fen Jia,Jian Guo Hao,Ying Juan Wang,Shu Zhen Feng,

The encoding sequence of gus gene from Escherichia coli was fused with maize Ubi-1 promoter and was introduced into maize genome via particle bombardment. Fertile transgenic maize plants were regenerated from bombarded type-I calluses which were derived from scutellar tissue of immature embryos based on PPT selection. Expression activity of gus gene under the control of Ubi-1 promoter was analysed using histochemical method, and the results showed that gus gene expressed in most tissues except anther. Ubi-GUS expression in pollen, egg cell and T1 immature embryos revealed that this promoter was active in early stages of plant development. Histochemically stained pollen grains of T0 plants showed a 1:1 segregation of the gus gene, which suggested that the foreign gene was inherited in Mendelian model in these plants. In addition, maize Ubi-1 promoter could reduce the copy number of foreign genes in transgenic maize plants, which might be useful in avoidance of gene silencing. T1 seeds have been harvested.

[Expression activity of maize Ubi-1 promoter in fertile transgenic maize plants] Publishing Authors By Initials

ZQ Xu,JF Jia,JG Hao,YJ Wang,SZ Feng,

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[Expression activity of maize Ubi-1 promoter in fertile transgenic maize plants] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Shi yan sheng wu xue bao

VOLUME: 35

Page Numbers: 296-302

Journal Abbreviation: Shi Yan Sheng Wu Xue Bao

ISSN: 0001-5334

DAY: 6

MONTH: Dec

YEAR: 2002

[Expression activity of maize Ubi-1 promoter in fertile transgenic maize plants] Information

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LANGUAGE: chi

NlmUniqueID: 413570

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AFFILIATION: Provincial Key Laboratory of Biotechnology, Northwest University, Xian 710069.

Country: China

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MEDLINETA: Shi Yan Sheng Wu Xue Bao

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