Exposure of bovine embryos to trypsin during washing does not decrease embryonic survival.

Exposure of bovine embryos to trypsin during washing does not decrease embryonic survival. Research Abstract Details 

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Exposure of bovine embryos to trypsin during washing does not decrease embryonic survival. Abstract Text:

S E Echternkamp,S M Kappes,R R Maurer,

The objective of this study was to assess whether the exposure of zona pellucida-intact bovine embryos to the proteolytic enzyme, trypsin, during embryo washing has a detrimental effect on their subsequent survival and development. Embryos were collected nonsurgically from superovulated cows (n = 19) 7.5 d after insemination. Grade 1 and Grade 2 embryos were washed 12 times in modified Dulbecco's phosphate buffered saline (PBS) containing 0.4% bovine serum albumin (BSA), or in a series of five washes in BSA-PBS (without Ca++ and Mg++), two in 0.25% trypsin in Hank's solution (without Ca++ and Mg++) and five in PBS-BSA medium. Within 30 min after washing, embryos were either transferred nonsurgically into recipient cows, 7 to 8 d post estrus, or cryopreserved and transferred later. Frozen-thawed embryos from five of the donors were cultured for 72 h in vitro and their development was evaluated. Pregnancy rates did not differ (P>0.1) between recipient cows receiving control-washed and trypsin-washed embryos transferred fresh (51.0 vs 56.3%). However, pregnancy rates were higher (P<0.05) for frozen-thawed embryos treated with trypsin before cryopreservation than for frozen-thawed, control-washed embryos (68.2 vs 38.5%). Survival and development of embryos in vitro after cryopreservation did not differ between embryos subjected to the control- and trypsin-wash procedures. These results suggest that exposure of bovine embryos to trypsin for 2 to 3 min during washing did not have a detrimental effect on embryonic development, but may have enhanced cryopreservation of the embryos.

Exposure of bovine embryos to trypsin during washing does not decrease embryonic survival. Publishing Authors By Initials

SE Echternkamp,SM Kappes,RR Maurer,

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Exposure of bovine embryos to trypsin during washing does not decrease embryonic survival. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Theriogenology

VOLUME: 32

Page Numbers: 131-7

Journal Abbreviation: Theriogenology

ISSN: 0093-691X

DAY: 26

MONTH: Jul

YEAR: 1989

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LANGUAGE: eng

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AFFILIATION: USDA, ARS, NPA, Roman L. Hruska U.S. Meat Animal Research Center Clay Center, NE 68933 USA.

Country: United States

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MEDLINETA: Theriogenology

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