Exploring the origins of cementoblasts and their trigger factors.

Exploring the origins of cementoblasts and their trigger factors. Research Abstract Details 

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Exploring the origins of cementoblasts and their trigger factors. Abstract Text:

Janice E Berry,Ming Zhao,Qiming Jin,Brian L Foster,Hema Viswanathan,Martha J Somerman,

Future directions for designing periodontal regenerative therapies hinge on characterization of cells associated with periodontal tissues and on signals instrumental in regulating their behavior. Studies here focused on determining the responsiveness of putative cementoblast progenitor cells (follicle cells), periodontal ligament cells, and cementoblasts to specific factors. Cementoblasts (OCCM), periodontal ligament (SVPDL) cells, and follicle cells (SVF4) were isolated from mouse tissues and immortalized, where SV indicates the use of SV-40 for immortalization. Cultured cells were examined for response to parathyroid hormone-related protein (PTHrP), bone morphogenetic protein-2 (BMP-2), and enamel matrix derivative (EMD) using RT-PCR, Northern analysis, and in vitro and in vivo mineralization assays. Results showed that the three cell lines varied in their expression of bone sialoprotein (BSP) and osteocalcin (OCN), and in their capacity to promote mineralization. RT-PCR indicated that all cell lines had PTH/PTHrP-R1 and BMP receptors; however, the response of individual cell lines to PTHrP, BMP-2, and EMD was quite different. Such dissimilarities in responsiveness associated with cell type may need to be considered when designing regenerative therapies.

Exploring the origins of cementoblasts and their trigger factors. Publishing Authors By Initials

JE Berry,M Zhao,Q Jin,BL Foster,H Viswanathan,MJ Somerman,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

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Exploring the origins of cementoblasts and their trigger factors. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Connective tissue research

VOLUME: 44 Suppl 1

Page Numbers: 97-102

Journal Abbreviation: Connect. Tissue Res.

ISSN: 0300-8207

DAY: 19

MONTH: 11

YEAR: 2003

Exploring the origins of cementoblasts and their trigger factors. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 365263

Exploring the origins of cementoblasts and their trigger factors. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: metabolism

Chemical & Substance for Abstract: Exploring the origins of cementoblasts and their trigger factors. Information

Substance Name: Osteocalcin

Registry Number: 104982-03-8

Grant and Affiliation Information for Exploring the origins of cementoblasts and their trigger factors.

AFFILIATION: Department of Periodontics/Prevention/Geriatrics, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48109-1078, USA. jeberry@umich.edu

Country: United States

AGENCY: United States NIDCR

GRANT: DE09532

ACRONYM: DE

MEDLINETA: Connect Tissue Res

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