Exploring strategies for protein trapping in Drosophila.

Exploring strategies for protein trapping in Drosophila. Research Abstract Details 

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Exploring strategies for protein trapping in Drosophila. Abstract Text:

Ana T ,Lisa N Petrella,Kathleen Ayers,Anthony Melillo,Stacy Mazzalupo,Andrew M Hudson,Shu Wang,Claudia Castiblanco,Michael Buszczak,Roger A Hoskins,Lynn Cooley,

The use of fluorescent protein tags has had a huge impact on cell biological studies in virtually every experimental system. Incorporation of coding sequence for fluorescent proteins such as green fluorescent protein (GFP) into genes at their endogenous chromosomal position is especially useful for generating GFP-fusion proteins that provide accurate cellular and subcellular expression data. We tested modifications of a transposon-based protein trap screening procedure in Drosophila to optimize the rate of recovering useful protein traps and their analysis. Transposons carrying the GFP-coding sequence flanked by splice acceptor and donor sequences were mobilized, and new insertions that resulted in production of GFP were captured using an automated embryo sorter. Individual stocks were established, GFP expression was analyzed during oogenesis, and insertion sites were determined by sequencing genomic DNA flanking the insertions. The resulting collection includes lines with protein traps in which GFP was spliced into mRNAs and embedded within endogenous proteins or enhancer traps in which GFP expression depended on splicing into transposon-derived RNA. We report a total of 335 genes associated with protein or enhancer traps and a web-accessible database for viewing molecular information and expression data for these genes.

Exploring strategies for protein trapping in Drosophila. Publishing Authors By Initials

AT ,LN Petrella,K Ayers,A Melillo,S Mazzalupo,AM Hudson,S Wang,C Castiblanco,M Buszczak,RA Hoskins,L Cooley,

For similar investigative techniques: genetic techniques: sequence analysis: sequence analysis, dna research abstracts see: investigative techniques: genetic techniques: sequence analysis: sequence analysis, dna research

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Exploring strategies for protein trapping in Drosophila. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Genetics

VOLUME: 175

Page Numbers: 1089-104

Journal Abbreviation: Genetics

ISSN: 0016-6731

DAY: 18

MONTH: 12

YEAR: 2006

Exploring strategies for protein trapping in Drosophila. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 374636

Exploring strategies for protein trapping in Drosophila. Keywords Mesh Terms:

KEYWORDS: Sequence Analysis, DNA

MESH TERMS: metabolism

Chemical & Substance for Abstract: Exploring strategies for protein trapping in Drosophila. Information

Substance Name: Green Fluorescent Proteins

Registry Number: 147336-22-9

Grant and Affiliation Information for Exploring strategies for protein trapping in Drosophila.

AFFILIATION: Department of Genetics, Cellular and Developmental Biology, Yale University School of Medicine, New Haven, Connecticut 06520, USA.

Country: United States

AGENCY: United States NCRR

GRANT: RR16749

ACRONYM: RR

MEDLINETA: Genetics

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