Experimental test of connector rotation during DNA packaging into bacteriophage phi29 capsids.

Experimental test of connector rotation during DNA packaging into bacteriophage phi29 capsids. Research Abstract Details 

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Experimental test of connector rotation during DNA packaging into bacteriophage phi29 capsids. Abstract Text:

Thorsten Hugel,Jens Michaelis,Craig L Hetherington,Paul J Jardine,Shelley Grimes,Jessica M Walter,Wayne Falk,Dwight L Anderson,Carlos Bustamante,

The bacteriophage phi29 generates large forces to compact its double-stranded DNA genome into a protein capsid by means of a portal motor complex. Several mechanical models for the generation of these high forces by the motor complex predict coupling of DNA translocation to rotation of the head-tail connector dodecamer. Putative connector rotation is investigated here by combining the methods of single-molecule force spectroscopy with polarization-sensitive single-molecule fluorescence. In our experiment, we observe motor function in several packaging complexes in parallel using video microscopy of bead position in a magnetic trap. At the same time, we follow the orientation of single fluorophores attached to the portal motor connector. From our data, we can exclude connector rotation with greater than 99% probability and therefore answer a long-standing mechanistic question.

Experimental test of connector rotation during DNA packaging into bacteriophage phi29 capsids. Publishing Authors By Initials

T Hugel,J Michaelis,CL Hetherington,PJ Jardine,S Grimes,JM Walter,W Falk,DL Anderson,C Bustamante,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: molecular structure: molecular conformation: nucleic acid conformation research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: molecular structure: molecular conformation: nucleic acid conformation research

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Experimental test of connector rotation during DNA packaging into bacteriophage phi29 capsids. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: PLoS biology

VOLUME: 5

Page Numbers: e59

Journal Abbreviation: PLoS Biol.

ISSN: 1545-7885

DAY: 3

MONTH: Mar

YEAR: 2007

Experimental test of connector rotation during DNA packaging into bacteriophage phi29 capsids. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101183755

Experimental test of connector rotation during DNA packaging into bacteriophage phi29 capsids. Keywords Mesh Terms:

KEYWORDS: Nucleic Acid Conformation

MESH TERMS: genetics

Chemical & Substance for Abstract: Experimental test of connector rotation during DNA packaging into bacteriophage phi29 capsids. Information

Substance Name: DNA, Viral

Registry Number: 0

Grant and Affiliation Information for Experimental test of connector rotation during DNA packaging into bacteriophage phi29 capsids.

AFFILIATION: Department of Physics, Technical University, Munich, Germany.

Country: United States

AGENCY: United States NIGMS

GRANT: GM-071552

ACRONYM: GM

MEDLINETA: PLoS Biol

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