Expansion of chromosome territories with chromatin decompaction in BAF53-depleted interphase cells.

Expansion of chromosome territories with chromatin decompaction in BAF53-depleted interphase cells. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Expansion of chromosome territories with chromatin decompaction in BAF53-depleted interphase cells. Abstract Text:

Kiwon Lee,Mi Jin Kang,Su Jin Kwon,Yunhee Kim Kwon,Ki Woo Kim,Jae-Hwan Lim,Hyockman Kwon,

Chromosomes are compartmentalized into discrete chromosome territories during interphase in mammalian cells. A chromosome territory is generated by the tendency of chromatin to occupy the smallest shell volume, which is determined by the polymeric properties and interactions of the internal meshwork of the chromatin fiber. Here, we show that BAF53 knockdown by small interfering RNA interference led to the expansion of chromosome territories. This was accompanied by a reduction in chromatin compaction, an increase in the micrococcal nuclease sensitivity of the chromatin, and an alteration in H3-K9 and H3-K79 dimethylation. Interestingly, the BAF53 knockdown cells suffer a cell cycle defect. Despite the significant irregularity and decompaction of the polynucleosomes isolated from the BAF53 knockdown cells, the chromatin loading of H1 and core histones remained unaltered, as did the nucleosome spacing. The histone hyperacetylation and down-regulation of BRG-1, mBrm, and Tip49, the catalytic components of the SWI/SNF complex and the TIP60 complex, respectively, did not expand chromosome territories. These results indicate that BAF53 contributes to the polymeric properties and/or the internal meshwork interactions of the chromatin fiber probably via a novel mechanism.

Expansion of chromosome territories with chromatin decompaction in BAF53-depleted interphase cells. Publishing Authors By Initials

K Lee,MJ Kang,SJ Kwon,YK Kwon,KW Kim,JH Lim,H Kwon,

For similar genetic processes: gene expression regulation: up-regulation research abstracts see: genetic processes: gene expression regulation: up-regulation research

PUBMED ID PMID:

MEDLINE DATE:

Expansion of chromosome territories with chromatin decompaction in BAF53-depleted interphase cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Molecular biology of the cell

VOLUME: 18

Page Numbers: 4013-23

Journal Abbreviation: Mol. Biol. Cell

ISSN: 1059-1524

DAY: 25

MONTH: 07

YEAR: 2007

Expansion of chromosome territories with chromatin decompaction in BAF53-depleted interphase cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9201390

Expansion of chromosome territories with chromatin decompaction in BAF53-depleted interphase cells. Keywords Mesh Terms:

KEYWORDS: Up-Regulation

MESH TERMS: genetics

Chemical & Substance for Abstract: Expansion of chromosome territories with chromatin decompaction in BAF53-depleted interphase cells. Information

Substance Name: Smarca4 protein, mouse

Registry Number: EC 3.6.1.-

Grant and Affiliation Information for Expansion of chromosome territories with chromatin decompaction in BAF53-depleted interphase cells.

AFFILIATION: Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, Yongin 449-791, Korea.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Mol Biol Cell

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Expansion of chromosome territories with chromatin decompaction in BAF53-depleted interphase cells Related Publications

• A new approach for the analysis of testicular cells using a laser scanning cytometer.
• A new curved rigid layngoscope to overcome the difficult laryngeal exposure (DLE) in endolaryngeal surgery.
• Amelioration of mercury-induced autoimmunity by 4-1BB.
• Automated segmentation of the optic disc from stereo color photographs using physiologically plausible features.
• B7-H3 ligand expression by prostate cancer: a novel marker of prognosis and potential target for therapy.
• Biochemical and genetic studies of UBR3, a ubiquitin ligase with a function in olfactory and other sensory systems.
• Biotin-triggered release of poly(ethylene glycol)-avidin from biotinylated polyethylenimine enhances in vitro gene expression.
• Blockade of the 4-1BB (CD137)/4-1BBL and/or CD28/CD80/CD86 costimulatory pathways promotes corneal allograft survival in mice.
• Comparative analysis of the effects of rice and bread meals on bioavailability of itraconazole using NONMEM in healthy volunteers.
• Downregulation of renal TonEBP in hypokalemic rats.
• Downregulation of renal sodium transporters and tonicity-responsive enhancer binding protein by long-term treatment with cyclosporin A.
• Glucocorticoid-induced tumour necrosis factor receptor family-related receptor signalling exacerbates hapten-induced colitis by CD4+ T cells.
• Hippocampal shape deformity analysis in obsessive-compulsive disorder.
• How tonicity regulates genes: story of TonEBP transcriptional activator.
• Lipophilic prodrugs of Hsp90 inhibitor geldanamycin for nanoencapsulation in poly(ethylene glycol)-b-poly(epsilon-caprolactone) micelles.
• Noise Analysis of MREIT at 3T and 11T Field Strength.
• Phosphine-catalyzed synthesis of highly functionalized coumarins.
• Poly(aspartate-g-PEI800), a polyethylenimine analogue of low toxicity and high transfection efficiency for gene delivery.
• Poly(ethylene glycol)-b-poly(epsilon-caprolactone) and PEG-phospholipid form stable mixed micelles in aqueous media.
• Polymeric micelles for drug delivery.
• Protein phosphatase 1 nuclear targeting subunit is a hypoxia inducible gene: its role in post-translational modification of p53 and MDM2.
• Resonance spectrum of a three-dimensional photonic quantum ring laser with an equilateral triangle microcavity.
• Simple and sensitive determination of menatetrenone and its epoxide metabolite in human plasma.
• Small-molecule inhibitors of protein geranylgeranyltransferase type I.
• Stable tetravalent phosphonium enolate zwitterions.
• Surgical procedure "simplified retrosigmoid approach" for C-P angle lesions.
• [Effect of endoscopic sclerotherapy using N-butyl-2-cyanoacrylate in patients with gastric variceal bleeding]
• [Effects of education on knowledge and practice of caregivers of the stroke patient]
• [Inflammatory pseudotumor of liver--a clinical review of 15 cases]
• [Interequipment variability of Doppler ultrasonographic indices in patients with liver cirrhosis]