Exo-beta-D-glucuronidase from the fungus Kobayasia nipponica.

Exo-beta-D-glucuronidase from the fungus Kobayasia nipponica. Research Abstract Details 

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Exo-beta-D-glucuronidase from the fungus Kobayasia nipponica. Abstract Text:

H Tsuchihashi,T Yadomae,T Miyazaki,

The occurrence of three forms, I, II, and III, of exo-beta-D-glucuronidase of the fungus Kobayasia nipponica was demonstrated. These three forms were purified 1,905-fold, 857-fold, and 357-fold, respectively. Forms I, II, and III of exo-beta-D-glucuronidase behaved differently on heparin-Sepharose chromatography, and differed in optimum pH (3.5, 3.2, and 2.6, respectively), pH-stability, Km (0.22, 0.16, and 0.13 mM, respectively), and Vmax (V) values. Their molecular weights, as estimated by gel filtration through Sephacryl S-200, were all 70,000; polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate gave a value of 72,000. These three forms were very active towards 1-4 linked beta-D-glucuronans, (Formula: see text) and (Formula: see text), but weak or inactive towards protuberic acid and several glycosaminoglycans.

Exo-beta-D-glucuronidase from the fungus Kobayasia nipponica. Publishing Authors By Initials

H Tsuchihashi,T Yadomae,T Miyazaki,

For similar carbohydrates: polysaccharides research abstracts see: carbohydrates: polysaccharides research

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Exo-beta-D-glucuronidase from the fungus Kobayasia nipponica. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 96

Page Numbers: 1789-97

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Dec

YEAR: 1984

Exo-beta-D-glucuronidase from the fungus Kobayasia nipponica. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Exo-beta-D-glucuronidase from the fungus Kobayasia nipponica. Keywords Mesh Terms:

KEYWORDS: Polysaccharides

MESH TERMS: metabolism

Chemical & Substance for Abstract: Exo-beta-D-glucuronidase from the fungus Kobayasia nipponica. Information

Substance Name: Glucuronidase

Registry Number: EC 3.2.1.31

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Country: JAPAN

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MEDLINETA: J Biochem

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