Excystation of Eimeria tenella Sporozoites Impaired by Antibody Recognizing Gametocyte/Oocyst Antigens GAM22 and GAM56.

Excystation of Eimeria tenella Sporozoites Impaired by Antibody Recognizing Gametocyte/Oocyst Antigens GAM22 and GAM56. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Excystation of Eimeria tenella Sporozoites Impaired by Antibody Recognizing Gametocyte/Oocyst Antigens GAM22 and GAM56. Abstract Text:

Jürgen Krücken,Ralf J Hosse,Aimdip N Mouafo,Rolf Entzeroth,Stefan Bierbaum,Predrag Marinovski,Karolina Hain,Gisela Greif,Frank Wunderlich,Jürgen Krücken,Ralf J Hosse,Aimdip N Mouafo,Rolf Entzeroth,Stefan Bierbaum,Predrag Marinovski,Karolina Hain,Gisela Greif,Frank Wunderlich, ,Ralf J Hosse,Aimdip N Mouafo,Rolf Entzeroth,Stefan Bierbaum,Predrag Marinovski,Karolina Hain,Gisela Greif,Frank Wunderlich,

Eimeria tenella is the causative agent of coccidiosis in poultry. Infection of the chicken intestine begins with ingestion of sporulated oocysts releasing sporocysts, which in turn release invasive sporozoites. The monoclonal antibody E2E5 recognizes wall-forming body type II (WFBII) in gametocytes and the WFBII-derived inner wall of oocysts. Here we describe that this antibody also binds to the stieda body of sporocysts and significantly impairs in vitro excystation of sporozoites. Using affinity chromatography and protein sequence analysis, E2E5 is shown to recognize EtGAM56, the E. tenella ortholog of the Eimeria maxima gametocyte-specific GAM56 protein. In addition, this antibody was used to screen a genomic phage display library presenting E. tenella antigens as fusion proteins with the gene VIII product on the surfaces of phagemid particles and identified the novel 22-kDa histidine- and proline-rich protein EtGAM22. The Etgam22 mRNA is expressed predominantly at the gametocyte stage, as detected by Northern blotting. Southern blot analysis in combination with data from the E. tenella genome project revealed that Etgam22 is an intronless multicopy gene, with approximately 12 to 22 copies in head-to-tail arrangement. Conspicuously, Etgam56 is also intronless and is localized adjacent to another gam56-like gene, Etgam59. Our data suggest that amplification is common for genes encoding oocyst wall proteins.

Excystation of Eimeria tenella Sporozoites Impaired by Antibody Recognizing Gametocyte/Oocyst Antigens GAM22 and GAM56. Publishing Authors By Initials

J Krücken,RJ Hosse,AN Mouafo,R Entzeroth,S Bierbaum,P Marinovski,K Hain,G Greif,F Wunderlich,J Krücken,RJ Hosse,AN Mouafo,R Entzeroth,S Bierbaum,P Marinovski,K Hain,G Greif,F Wunderlich,J ,RJ Hosse,AN Mouafo,R Entzeroth,S Bierbaum,P Marinovski,K Hain,G Greif,F Wunderlich,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Excystation of Eimeria tenella Sporozoites Impaired by Antibody Recognizing Gametocyte/Oocyst Antigens GAM22 and GAM56. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Eukaryotic cell

VOLUME: 7

Page Numbers: 202-11

Journal Abbreviation: Eukaryotic Cell

ISSN: 1535-9786

DAY: 14

MONTH: 12

YEAR: 2007

Excystation of Eimeria tenella Sporozoites Impaired by Antibody Recognizing Gametocyte/Oocyst Antigens GAM22 and GAM56. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101130731

Excystation of Eimeria tenella Sporozoites Impaired by Antibody Recognizing Gametocyte/Oocyst Antigens GAM22 and GAM56. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Excystation of Eimeria tenella Sporozoites Impaired by Antibody Recognizing Gametocyte/Oocyst Antigens GAM22 and GAM56. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Excystation of Eimeria tenella Sporozoites Impaired by Antibody Recognizing Gametocyte/Oocyst Antigens GAM22 and GAM56.

AFFILIATION: Division of Molecular Parasitology, Heinrich-Heine-University, Universitätsstr. 1, 40225 Düsseldorf, Germany. kruecken@uni-duesseldorf.de.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Eukaryot Cell

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Excystation of Eimeria tenella Sporozoites Impaired by Antibody Recognizing Gametocyte/Oocyst Antigens GAM22 and GAM56 Related Publications

• Beyond a relay nucleus: neuroimaging views on the human LGN.
• Effectiveness of postoperative adjuvant therapy with cytotoxic chemotherapy (cytosine arabinoside, mitomycin C, 5-fluorouracil) or immunotherapy (neuraminidase-modified allogeneic cells) in the prevention of recurrence of Duke's B and C colon cancer.
• Eimeria tenella: Genomic organization and expression of an 89kDa cyclophilin.
• Excystation of Eimeria tenella Sporozoites Impaired by Antibody Recognizing Gametocyte/Oocyst Antigens GAM22 and GAM56.
• Laser-supported partial nephrectomy for renal cell carcinoma.
• Mass transport parameters of aspen wood chip beds via stimulus-response tracer techniques.
• Mobile parametric sub-bottom profiler system for shallow and medium depth applications.
• Monomer Adsorption on Equilateral Triangular Lattices with Attractive First-neighbor Interactions.
• Stable five- and six-coordinate cobalt(III) complexes with a pentadentate bispidine ligand.
• Var transcription profiling of Plasmodium falciparum 3D7: assignment of cytoadherent phenotypes to dominant transcripts.
• ["Substances capable of self-reproduction" as cellular targets of chemical carcinogens]
• [Comment on the contribution by Ursula Geissler: "The non-puerperal secreting breast"]
• [Congenital total AV block in the direct fetal electrocardiogram]
• [Cytologic findings in ectopia of the decidua (deciduosis) of the portio vaginalis uteri]
• [Double prolapse of arms and legs in occiput position as a rare indication for cesarean section]
• [Extrasystole in the intranatal electrocardiogram]
• [Galactography in the diagnosis of breast cancer]
• [PQ interval and pre-excitation syndrome in direct fetal electrocardiogram--fetal Lown-Ganong-Levine (LGL) syndrome]
• [Partial hydatidiform mole with a full-term infant. (Case report)]
• [Passive electrical investigations on erythrocytes in dependence of age donars (author's transl)]
• [Possibilities for misinterpretation of direct fetal electrocardiography]
• [Prolapse of the small intestine through a surgically closed introitus vaginae after vaginal hysterectomy followed by subsequent colpectomy]
• [Quality Profile of a CAM Rehabilitation Clinic - Part 1: Structure, Quality Assurance and Interventions.]
• [Retroperitoneal malignant neurinoma (a case report)]
• [Serological studies on the occurrence and spread of reovirus infections in Bavarian pig herds]
• [The psychodiagnosis of the organically brain-damaged child.]
• [Therapy of fetal cardiac arrhythmia ante partum and sub partu]
• [Unusual form of genital malformation with aplasia of the right kidney]
• [Vaginaefixatio sacrospinalis vaginalis (Amreich II) for prolapse of vaginal stump (author's transl)]
• [What is the significance of the multi-hit theory of the viral origin of malignant tumors?]