Exceptional flexibility in the sequence requirements for coronavirus small envelope protein function.

Exceptional flexibility in the sequence requirements for coronavirus small envelope protein function. Research Abstract Details 

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Exceptional flexibility in the sequence requirements for coronavirus small envelope protein function. Abstract Text:

Lili Kuo,Kelley R Hurst,Paul S Masters,

The small envelope protein (E) plays a role of central importance in the assembly of coronaviruses. This was initially established by studies demonstrating that cellular expression of only E protein and the membrane protein (M) was necessary and sufficient for the generation and release of virus-like particles. To investigate the role of E protein in the whole virus, we previously generated E gene mutants of mouse hepatitis virus (MHV) that were defective in viral growth and produced aberrantly assembled virions. Surprisingly, however, we were also able to isolate a viable MHV mutant (DeltaE) in which the entire E gene, as well as the nonessential upstream genes 4 and 5a, were deleted. We have now constructed an E knockout mutant that confirms that the highly defective phenotype of the DeltaE mutant is due to loss of the E gene. Additionally, we have created substitution mutants in which the MHV E gene was replaced by heterologous E genes from viruses spanning all three groups of the coronavirus family. Group 2 and 3 E proteins were readily exchangeable for that of MHV. However, the E protein of a group 1 coronavirus, transmissible gastroenteritis virus, became functional in MHV only after acquisition of particular mutations. Our results show that proteins encompassing a remarkably diverse range of primary amino acid sequences can provide E protein function in MHV. These findings suggest that E protein facilitates viral assembly in a manner that does not require E protein to make sequence-specific contacts with M protein.

Exceptional flexibility in the sequence requirements for coronavirus small envelope protein function. Publishing Authors By Initials

L Kuo,KR Hurst,PS Masters,

For similar biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: viral physiology: virus replication research abstracts see: biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: viral physiology: virus replication research

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MEDLINE DATE:

Exceptional flexibility in the sequence requirements for coronavirus small envelope protein function. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of virology

VOLUME: 81

Page Numbers: 2249-62

Journal Abbreviation: J. Virol.

ISSN: 0022-538X

DAY: 20

MONTH: 12

YEAR: 2006

Exceptional flexibility in the sequence requirements for coronavirus small envelope protein function. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 113724

Exceptional flexibility in the sequence requirements for coronavirus small envelope protein function. Keywords Mesh Terms:

KEYWORDS: Virus Replication

MESH TERMS: physiology

Chemical & Substance for Abstract: Exceptional flexibility in the sequence requirements for coronavirus small envelope protein function. Information

Substance Name: Viral Envelope Proteins

Registry Number: 0

Grant and Affiliation Information for Exceptional flexibility in the sequence requirements for coronavirus small envelope protein function.

AFFILIATION: David Axelrod Institute, Wadsworth Center, NY State Department of Health, New Scotland Avenue, Albany, NY 12201-2002, USA. masters@wadsworth.org

Country: United States

AGENCY: United States NIAID

GRANT: AI 064603

ACRONYM: AI

MEDLINETA: J Virol

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