Examination of Pseudomonas aeruginosa lasI regulation and 3-oxo-C12-homoserine lactone production using a heterologous Escherichia coli system.

Examination of Pseudomonas aeruginosa lasI regulation and 3-oxo-C12-homoserine lactone production using a heterologous Escherichia coli system. Research Abstract Details 

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Examination of Pseudomonas aeruginosa lasI regulation and 3-oxo-C12-homoserine lactone production using a heterologous Escherichia coli system. Abstract Text:

Matthew J Wargo,Deborah A Hogan,

In Pseudomonas aeruginosa, the signaling molecule 3-oxo-C12-homoserine lactone (3OC12HSL) is synthesized by LasI, and lasI transcription is positively regulated by LasR. A heterologous model has been generated for the study of the LasRI/3OC12HSL regulatory network in Escherichia coli. Escherichia coli pAHL-BAC cultures produced LasI-synthesized acylhomoserine lactones (AHLs) at levels and with kinetics similar to what is observed in cultures of P. aeruginosa strain PAO1. Analysis of the lasI transcript also showed similar induction profiles in both the E. coli pAHL-BAC strain and P. aeruginosa. Transposon mutagenesis of pAHL-BAC confirmed that transcriptional regulation by LasR is necessary for 3OC12HSL production, and showed that artificially increasing lasI transcript levels leads to higher levels of 3OC12HSL. Previous studies have shown that P. aeruginosa 3OC12HSL inhibits hypha formation, but not growth, in Candida albicans, and the E. coli pAHL-BAC similarly inhibited filamentation when grown in coculture with the fungus. It is proposed that this system will be useful for the study of factors that impact lasI regulation and 3OC12HSL production, and for the examination of the role of LasI-produced AHLs in bacterial interactions with other organisms.

Examination of Pseudomonas aeruginosa lasI regulation and 3-oxo-C12-homoserine lactone production using a heterologous Escherichia coli system. Publishing Authors By Initials

MJ Wargo,DA Hogan,

For similar genetic processes: gene expression: transcription, genetic research abstracts see: genetic processes: gene expression: transcription, genetic research

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Examination of Pseudomonas aeruginosa lasI regulation and 3-oxo-C12-homoserine lactone production using a heterologous Escherichia coli system. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: FEMS microbiology letters

VOLUME: 273

Page Numbers: 38-44

Journal Abbreviation: FEMS Microbiol. Lett.

ISSN: 0378-1097

DAY: 7

MONTH: 06

YEAR: 2007

Examination of Pseudomonas aeruginosa lasI regulation and 3-oxo-C12-homoserine lactone production using a heterologous Escherichia coli system. Information

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LANGUAGE: eng

NlmUniqueID: 7705721

Examination of Pseudomonas aeruginosa lasI regulation and 3-oxo-C12-homoserine lactone production using a heterologous Escherichia coli system. Keywords Mesh Terms:

KEYWORDS: Transcription, Genetic

MESH TERMS: physiology

Chemical & Substance for Abstract: Examination of Pseudomonas aeruginosa lasI regulation and 3-oxo-C12-homoserine lactone production using a heterologous Escherichia coli system. Information

Substance Name: 4-Butyrolactone

Registry Number: 96-48-0

Grant and Affiliation Information for Examination of Pseudomonas aeruginosa lasI regulation and 3-oxo-C12-homoserine lactone production using a heterologous Escherichia coli system.

AFFILIATION: Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, NH 03755, USA.

Country: England

AGENCY: United States NIAID

GRANT: T32 AI07519

ACRONYM: AI

MEDLINETA: FEMS Microbiol Lett

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