EXAFS reveals a structural zinc binding site in the bovine NADH-Q oxidoreductase.

EXAFS reveals a structural zinc binding site in the bovine NADH-Q oxidoreductase. Research Abstract Details 

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EXAFS reveals a structural zinc binding site in the bovine NADH-Q oxidoreductase. Abstract Text:

Lisa Giachini,Francesco Francia,Federico Boscherini,Consiglia Pacelli,Tiziana Cocco,Sergio Papa,Giovanni Venturoli,Lisa Giachini,Francesco Francia,Federico Boscherini,Consiglia Pacelli,Tiziana Cocco,Sergio Papa,Giovanni Venturoli,Lisa Giachini,Francesco Francia,Federico Boscherini,Consiglia Pacelli,Tiziana Cocco,Sergio Papa,Giovanni Venturoli,

The metal content of bovine NADH-Q oxidoreductase determined by inductively-coupled plasma atomic-emission spectroscopy reveals the presence of about one atom of zinc per molecule of flavin mononucleotide. We applied Zn K-edge extended X-ray absorption fine structure spectroscopy (EXAFS) to investigate the local structure of the bound zinc ion and to identify the nature of the coordinating residues. The EXAFS spectrum is consistent with a structured zinc binding site. By combining information from first-shell analysis and from metalloprotein data bases putative binding clusters have been built and fitted to the experimental spectrum using ab initio simulations. The best fitting binding cluster is formed by two histidine and two cysteine residues arranged in a tetrahedral geometry.

EXAFS reveals a structural zinc binding site in the bovine NADH-Q oxidoreductase. Publishing Authors By Initials

L Giachini,F Francia,F Boscherini,C Pacelli,T Cocco,S Papa,G Venturoli,L Giachini,F Francia,F Boscherini,C Pacelli,T Cocco,S Papa,G Venturoli,L Giachini,F Francia,F Boscherini,C Pacelli,T Cocco,S Papa,G Venturoli,

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EXAFS reveals a structural zinc binding site in the bovine NADH-Q oxidoreductase. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: FEBS letters

VOLUME: 581

Page Numbers: 5645-8

Journal Abbreviation: FEBS Lett.

ISSN: 0014-5793

DAY: 20

MONTH: 11

YEAR: 2007

EXAFS reveals a structural zinc binding site in the bovine NADH-Q oxidoreductase. Information

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LANGUAGE: eng

NlmUniqueID: 155157

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AFFILIATION: Department of Biology, University of Bologna, Via Irnerio n 42, 40126, Bologna, Italy.

Country: Netherlands

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MEDLINETA: FEBS Lett

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