Evolutionarily conserved multisubunit RBL2/p130 and E2F4 protein complex represses human cell cycle-dependent genes in quiescence.

Evolutionarily conserved multisubunit RBL2/p130 and E2F4 protein complex represses human cell cycle-dependent genes in quiescence. Research Abstract Details 

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Evolutionarily conserved multisubunit RBL2/p130 and E2F4 protein complex represses human cell cycle-dependent genes in quiescence. Abstract Text:

Larisa Litovchick,Subhashini Sadasivam,Laurence Florens,Xiaopeng Zhu,Selene K Swanson,Soundarapandian Velmurugan,Runsheng Chen,Michael P Washburn,X Shirley Liu,James A DeCaprio,

The mammalian Retinoblastoma (RB) family including pRB, p107, and p130 represses E2F target genes through mechanisms that are not fully understood. In D. melanogaster, RB-dependent repression is mediated in part by the multisubunit protein complex Drosophila RBF, E2F, and Myb (dREAM) that contains homologs of the C. elegans synthetic multivulva class B (synMuvB) gene products. Using an integrated approach combining proteomics, genomics, and bioinformatic analyses, we identified a p130 complex termed DP, RB-like, E2F, and MuvB (DREAM) that contains mammalian homologs of synMuvB proteins LIN-9, LIN-37, LIN-52, LIN-54, and LIN-53/RBBP4. DREAM bound to more than 800 human promoters in G0 and was required for repression of E2F target genes. In S phase, MuvB proteins dissociated from p130 and formed a distinct submodule that bound MYB. This work reveals an evolutionarily conserved multisubunit protein complex that contains p130 and E2F4, but not pRB, and mediates the repression of cell cycle-dependent genes in quiescence.

Evolutionarily conserved multisubunit RBL2/p130 and E2F4 protein complex represses human cell cycle-dependent genes in quiescence. Publishing Authors By Initials

L Litovchick,S Sadasivam,L Florens,X Zhu,SK Swanson,S Velmurugan,R Chen,MP Washburn,XS Liu,JA DeCaprio,

For similar genetic processes: mutagenesis: suppression, genetic research abstracts see: genetic processes: mutagenesis: suppression, genetic research

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Evolutionarily conserved multisubunit RBL2/p130 and E2F4 protein complex represses human cell cycle-dependent genes in quiescence. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Molecular cell

VOLUME: 26

Page Numbers: 539-51

Journal Abbreviation: Mol. Cell

ISSN: 1097-2765

DAY: 25

MONTH: May

YEAR: 2007

Evolutionarily conserved multisubunit RBL2/p130 and E2F4 protein complex represses human cell cycle-dependent genes in quiescence. Information

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LANGUAGE: eng

NlmUniqueID: 9802571

Evolutionarily conserved multisubunit RBL2/p130 and E2F4 protein complex represses human cell cycle-dependent genes in quiescence. Keywords Mesh Terms:

KEYWORDS: Suppression, Genetic

MESH TERMS: metabolism

Chemical & Substance for Abstract: Evolutionarily conserved multisubunit RBL2/p130 and E2F4 protein complex represses human cell cycle-dependent genes in quiescence. Information

Substance Name: Retinoblastoma-Like Protein p130

Registry Number: 0

Grant and Affiliation Information for Evolutionarily conserved multisubunit RBL2/p130 and E2F4 protein complex represses human cell cycle-dependent genes in quiescence.

AFFILIATION: Department of Medical Oncology, Dana-Farber Cancer Institute and Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 44 Binney Street, Boston, MA 02115, USA.

Country: United States

AGENCY: United States NCI

GRANT: R01 CA063113

ACRONYM: CA

MEDLINETA: Mol Cell

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