Evolution of new nonantibody proteins via iterative somatic hypermutation.

Evolution of new nonantibody proteins via iterative somatic hypermutation. Research Abstract Details 

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Evolution of new nonantibody proteins via iterative somatic hypermutation. Abstract Text:

Lei Wang,W Coyt Jackson,Paul A Steinbach,Roger Y Tsien,

B lymphocytes use somatic hypermutation (SHM) to optimize immunoglobulins. Although SHM can rescue single point mutations deliberately introduced into nonimmunoglobulin genes, such experiments do not show whether SHM can efficiently evolve challenging novel phenotypes requiring multiple unforeseeable mutations in nonantibody proteins. We have now iterated SHM over 23 rounds of fluorescence-activated cell sorting to create monomeric red fluorescent proteins with increased photostability and far-red emissions (e.g., 649 nm), surpassing the best efforts of structure-based design. SHM offers a strategy to evolve nonantibody proteins with desirable properties for which a high-throughput selection or viable single-cell screen can be devised.

Evolution of new nonantibody proteins via iterative somatic hypermutation. Publishing Authors By Initials

L Wang,WC Jackson,PA Steinbach,RY Tsien,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: sequence homology: sequence homology, amino acid research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: sequence homology: sequence homology, amino acid research

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Evolution of new nonantibody proteins via iterative somatic hypermutation. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 101

Page Numbers: 16745-9

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 0027-8424

DAY: 19

MONTH: 11

YEAR: 2004

Evolution of new nonantibody proteins via iterative somatic hypermutation. Information

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LANGUAGE: eng

NlmUniqueID: 7505876

Evolution of new nonantibody proteins via iterative somatic hypermutation. Keywords Mesh Terms:

KEYWORDS: Sequence Homology, Amino Acid

MESH TERMS: genetics

Chemical & Substance for Abstract: Evolution of new nonantibody proteins via iterative somatic hypermutation. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Evolution of new nonantibody proteins via iterative somatic hypermutation.

AFFILIATION: Department of Pharmacology, University of California at San Diego, La Jolla, CA 92093-0647, USA.

Country: United States

AGENCY: United States NINDS

GRANT: NS27177

ACRONYM: NS

MEDLINETA: Proc Natl Acad Sci U S A

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