Evolution of a fluorinated green fluorescent protein.

Evolution of a fluorinated green fluorescent protein. Research Abstract Details 

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Evolution of a fluorinated green fluorescent protein. Abstract Text:

Tae Hyeon Yoo,A James Link,David A Tirrell,

The fluorescence of bacterial cells expressing a variant (GFPm) of the green fluorescent protein (GFP) was reduced to background levels by global replacement of the leucine residues of GFPm by 5,5,5-trifluoroleucine. Eleven rounds of random mutagenesis and screening via fluorescence-activated cell sorting yielded a GFP mutant containing 20 amino acid substitutions. The mutant protein in fluorinated form showed improved folding efficiency both in vivo and in vitro, and the median fluorescence of cells expressing the fluorinated protein was improved approximately 650-fold in comparison to that of cells expressing fluorinated GFPm. The success of this approach demonstrates the feasibility of engineering functional proteins containing many copies of abiological amino acid constituents.

Evolution of a fluorinated green fluorescent protein. Publishing Authors By Initials

TH Yoo,AJ Link,DA Tirrell,

For similar genetic phenomena: variation (genetics) research abstracts see: genetic phenomena: variation (genetics) research

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Evolution of a fluorinated green fluorescent protein. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 104

Page Numbers: 13887-90

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 0027-8424

DAY: 23

MONTH: 08

YEAR: 2007

Evolution of a fluorinated green fluorescent protein. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7505876

Evolution of a fluorinated green fluorescent protein. Keywords Mesh Terms:

KEYWORDS: Variation (Genetics)

MESH TERMS: metabolism

Chemical & Substance for Abstract: Evolution of a fluorinated green fluorescent protein. Information

Substance Name: Green Fluorescent Proteins

Registry Number: 147336-22-9

Grant and Affiliation Information for Evolution of a fluorinated green fluorescent protein.

AFFILIATION: Division of Chemistry and Chemical Engineering, Joseph J. Jacobs Institute for Molecular Engineering for Medicine, California Institute of Technology, 1200 East California Boulevard, MC210-41, Pasadena, CA 91125-4100, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM62523

ACRONYM: GM

MEDLINETA: Proc Natl Acad Sci U S A

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