Evidence that the heparin-binding consensus sequence of vitronectin is recognized by Staphylococcus aureus.

Evidence that the heparin-binding consensus sequence of vitronectin is recognized by Staphylococcus aureus. Research Abstract Details 

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Evidence that the heparin-binding consensus sequence of vitronectin is recognized by Staphylococcus aureus. Abstract Text:

O D Liang,J I Flock,T ,

Binding of heparin-binding form of vitronectin to Staphylococcus aureus was inhibited completely by heparin or by the same form of vitronectin. The binding was inhibited only to about 50% by the non-heparin-binding form of vitronectin, indicating an apparent involvement of the heparin-binding properties in the interaction between vitronectin and S. aureus. This was supported by experiments in which a synthetic peptide (Ala347-Arg361, comprising heparin-binding consensus sequences) was found to partly inhibit bacterial adherence to immobilized vitronectin. A bacterial cell surface protein could bind to the quinquedecapeptide, but not to the highly charged peptides consisting entirely of arginine or lysine, immobilized on microtiter plates and the binding could be competitively inhibited by an excess of soluble peptide. Direct binding of radiolabeled peptide to bacterial cells was also demonstrated, which was rapid, saturable, and pH-dependent. Furtherly a bacterial surface protein having molecular mass of 60 kDa was isolated by affinity chromatography on a quinquedecapeptide-HiTrap-NHS column. Our data suggest that the heparin-binding properties of vitronectin play a role in bacterial recognition.

Evidence that the heparin-binding consensus sequence of vitronectin is recognized by Staphylococcus aureus. Publishing Authors By Initials

OD Liang,JI Flock,T ,

For similar proteins: blood proteins: vitronectin research abstracts see: proteins: blood proteins: vitronectin research

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Evidence that the heparin-binding consensus sequence of vitronectin is recognized by Staphylococcus aureus. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 116

Page Numbers: 457-63

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Aug

YEAR: 1994

Evidence that the heparin-binding consensus sequence of vitronectin is recognized by Staphylococcus aureus. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Evidence that the heparin-binding consensus sequence of vitronectin is recognized by Staphylococcus aureus. Keywords Mesh Terms:

KEYWORDS: Vitronectin

MESH TERMS: metabolism

Chemical & Substance for Abstract: Evidence that the heparin-binding consensus sequence of vitronectin is recognized by Staphylococcus aureus. Information

Substance Name: Heparin

Registry Number: 9005-49-6

Grant and Affiliation Information for Evidence that the heparin-binding consensus sequence of vitronectin is recognized by Staphylococcus aureus.

AFFILIATION: Department of Medical Microbiology, University of Lund, Sweden.

Country: JAPAN

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MEDLINETA: J Biochem

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