Evidence of post-transcriptional regulation in the maintenance of a partial muscle phenotype by electrogenic cells of S. macrurus.

Evidence of post-transcriptional regulation in the maintenance of a partial muscle phenotype by electrogenic cells of S. macrurus. Research Abstract Details 

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Evidence of post-transcriptional regulation in the maintenance of a partial muscle phenotype by electrogenic cells of S. macrurus. Abstract Text:

Heriberto Cuellar,Jung A Kim,Graciela A Unguez,

Electrocytes, the current-producing cells of electric organs (EOs) in electric fish, are unique in that they derive from striated muscle and they possess biochemical characteristics of both muscle and non-muscle cells. In the freshwater teleost Sternopygus macrurus, electrocytes are multinucleated cells that do not contract yet retain expression of some proteins common to skeletal muscle cells. Given the role that transcriptional regulation plays in the activation of the myogenic program in vertebrates, we examined the expression patterns of several genes associated with multiple functions of skeletal muscle in mature electrocytes of S. macrurus. Our expression analyses detected transcripts for alpha-actin, alpha-acetylcholine (ACh) receptor (alpha-AChR), desmin, muscle creatine kinase (MCK), myosin heavy chain (MHC) isoforms, titin, tropomyosin, and troponin-T genes in the EO. However, immunolabeling studies revealed that electrocytes do not contain MCK, MHCs, or tropomyosin or troponin-T proteins. These results underscore the contribution of gene regulatory mechanisms in the maintenance of the muscle-like phenotype of EO that may be transcriptional-independent. We also report the classification and frequency of distinct transcripts from a random selection of 420 clones from an EO cDNA library. This is the first characterization of expressed genes in an EO, and it is an important step toward identifying mechanisms that affect different muscle protein systems for the evolution of highly specialized noncontractile tissues. Evidence of post-transcriptional regulation in the maintenance of a partial muscle phenotype by electrogenic cells of S. macrurus.

Evidence of post-transcriptional regulation in the maintenance of a partial muscle phenotype by electrogenic cells of S. macrurus. Publishing Authors By Initials

H Cuellar,JA Kim,GA Unguez,

For similar animal structures: tail research abstracts see: animal structures: tail research

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Evidence of post-transcriptional regulation in the maintenance of a partial muscle phenotype by electrogenic cells of S. macrurus. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The FASEB journal : official publication of the Fe

VOLUME: 20

Page Numbers: 2540

Journal Abbreviation: FASEB J.

ISSN: 1530-6860

DAY: 31

MONTH: 10

YEAR: 2006

Evidence of post-transcriptional regulation in the maintenance of a partial muscle phenotype by electrogenic cells of S. macrurus. Information

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LANGUAGE: eng

NlmUniqueID: 8804484

Evidence of post-transcriptional regulation in the maintenance of a partial muscle phenotype by electrogenic cells of S. macrurus. Keywords Mesh Terms:

KEYWORDS: Tail

MESH TERMS: metabolism

Chemical & Substance for Abstract: Evidence of post-transcriptional regulation in the maintenance of a partial muscle phenotype by electrogenic cells of S. macrurus. Information

Substance Name: Nuclear Proteins

Registry Number: 0

Grant and Affiliation Information for Evidence of post-transcriptional regulation in the maintenance of a partial muscle phenotype by electrogenic cells of S. macrurus.

AFFILIATION: Department of Biology, Foster Hall, New Mexico State University, Las Cruces, NM 88003, USA.

Country: United States

AGENCY: United States PHS

GRANT: S06-GMO8136-27

ACRONYM: RR

MEDLINETA: FASEB J

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