Evidence for structural constraint on ovulin, a rapidly evolving Drosophila melanogaster seminal protein.

Evidence for structural constraint on ovulin, a rapidly evolving Drosophila melanogaster seminal protein. Research Abstract Details 

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Evidence for structural constraint on ovulin, a rapidly evolving Drosophila melanogaster seminal protein. Abstract Text:

Alex Wong,Shannon N Albright,Mariana F Wolfner,

The egg-laying hormone ovulin (Acp26Aa) is among the most rapidly evolving proteins in the Drosophila genome. Against the background of ovulin's high sequence variability within and between species, we have identified highly conserved motifs that may play an important structural role. Using yeast two-hybrid and GST-pull-down assays, we show that ovulin interacts with itself. The C terminus of ovulin is necessary and sufficient for self-interaction, with its C-terminal 45 aa playing a major role. Under nonreducing conditions, ovulin participates in a high-molecular-mass complex, suggesting that it occurs in an oligomeric form. One or more of three predicted coiled-coil domains in the C terminus of ovulin may be involved in its self-interaction. These structural elements are conserved between species despite an overall rapid pace of evolution in ovulin's primary sequence. We therefore suggest that domains involved in ovulin's self-interaction form a conserved structural backbone for the protein, resulting in greater evolutionary flexibility at other sites.

Evidence for structural constraint on ovulin, a rapidly evolving Drosophila melanogaster seminal protein. Publishing Authors By Initials

A Wong,SN Albright,MF Wolfner,

For similar proteins: seminal plasma proteins research abstracts see: proteins: seminal plasma proteins research

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Evidence for structural constraint on ovulin, a rapidly evolving Drosophila melanogaster seminal protein. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 103

Page Numbers: 18644-9

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 0027-8424

DAY: 27

MONTH: 11

YEAR: 2006

Evidence for structural constraint on ovulin, a rapidly evolving Drosophila melanogaster seminal protein. Information

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LANGUAGE: eng

NlmUniqueID: 7505876

Evidence for structural constraint on ovulin, a rapidly evolving Drosophila melanogaster seminal protein. Keywords Mesh Terms:

KEYWORDS: Seminal Plasma Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Evidence for structural constraint on ovulin, a rapidly evolving Drosophila melanogaster seminal protein. Information

Substance Name: Seminal Plasma Proteins

Registry Number: 0

Grant and Affiliation Information for Evidence for structural constraint on ovulin, a rapidly evolving Drosophila melanogaster seminal protein.

AFFILIATION: Department of Molecular Biology and Genetics, Biotechnology Building Room 423, Cornell University, Ithaca, NY 14853, USA.

Country: United States

AGENCY: United States PHS

GRANT: T32 07617

ACRONYM: HD

MEDLINETA: Proc Natl Acad Sci U S A

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