Evidence for fast conformational change upon ligand dissociation in the HemAT class of bacterial oxygen sensors.

Evidence for fast conformational change upon ligand dissociation in the HemAT class of bacterial oxygen sensors. Research Abstract Details 

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Evidence for fast conformational change upon ligand dissociation in the HemAT class of bacterial oxygen sensors. Abstract Text:

Audrey Mokdad,Mark Nissen,James D Satterlee,Randy W Larsen,Audrey Mokdad,Mark Nissen,James D Satterlee,Randy W Larsen,

Here we report the results of transient absorption and photoacoustic calorimetry studies of CO photodissociation from the heme domain of the bacterial oxygen sensor HemAT-Bs. The results indicate that CO photolysis is accompanied by an overall DeltaH of -19 kcal mol(-1) and DeltaV of +4 ml mol(-1) as well as a red-shifted kinetic difference spectrum all occurring in <50 ns. Analysis of the DeltaH/DeltaV reveals that a conformational change takes place with a DeltaH(conf) of -40 kcal mol(-1) and DeltaV(conf) of -22 ml mol(-1). These thermodynamic changes are consistent with an increase in the solvent accessible surface area of the protein upon ligand dissociation, as observed in the X-ray structure of the ferric CN-bound and CN free forms of HemAT-Bs.

Evidence for fast conformational change upon ligand dissociation in the HemAT class of bacterial oxygen sensors. Publishing Authors By Initials

A Mokdad,M Nissen,JD Satterlee,RW Larsen,A Mokdad,M Nissen,JD Satterlee,RW Larsen,

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Evidence for fast conformational change upon ligand dissociation in the HemAT class of bacterial oxygen sensors. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: FEBS letters

VOLUME: 581

Page Numbers: 4512-8

Journal Abbreviation: FEBS Lett.

ISSN: 0014-5793

DAY: 24

MONTH: 08

YEAR: 2007

Evidence for fast conformational change upon ligand dissociation in the HemAT class of bacterial oxygen sensors. Information

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LANGUAGE: eng

NlmUniqueID: 155157

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Grant and Affiliation Information for Evidence for fast conformational change upon ligand dissociation in the HemAT class of bacterial oxygen sensors.

AFFILIATION: Department of Chemistry, University of South Florida, 4202 East Fowler Ave., Tampa, FL 33620, United States.

Country: Netherlands

AGENCY: United States NIGMS

GRANT: GM47645

ACRONYM: GM

MEDLINETA: FEBS Lett

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