Evidence for diversity in transcriptional profiles of single hematopoietic stem cells.

Evidence for diversity in transcriptional profiles of single hematopoietic stem cells. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Evidence for diversity in transcriptional profiles of single hematopoietic stem cells. Abstract Text:

Carlos A Ramos,Teresa A Bowman,Nathan C Boles,Akil A Merchant,Yayun Zheng,Irma Parra,Suzanne A W Fuqua,Chad A Shaw,Margaret A Goodell,

Hematopoietic stem cells replenish all the cells of the blood throughout the lifetime of an animal. Although thousands of stem cells reside in the bone marrow, only a few contribute to blood production at any given time. Nothing is known about the differences between individual stem cells that dictate their particular state of activation readiness. To examine such differences between individual stem cells, we determined the global gene expression profile of 12 single stem cells using microarrays. We showed that at least half of the genetic expression variability between 12 single cells profiled was due to biological variation in 44% of the genes analyzed. We also identified specific genes with high biological variance that are candidates for influencing the state of readiness of individual hematopoietic stem cells, and confirmed the variability of a subset of these genes using single-cell real-time PCR. Because apparent variation of some genes is likely due to technical factors, we estimated the degree of biological versus technical variation for each gene using identical RNA samples containing an RNA amount equivalent to that of single cells. This enabled us to identify a large cohort of genes with low technical variability whose expression can be reliably measured on the arrays at the single-cell level. These data have established that gene expression of individual stem cells varies widely, despite extremely high phenotypic homogeneity. Some of this variation is in key regulators of stem cell activity, which could account for the differential responses of particular stem cells to exogenous stimuli. The capacity to accurately interrogate individual cells for global gene expression will facilitate a systems approach to biological processes at a single-cell level.

Evidence for diversity in transcriptional profiles of single hematopoietic stem cells. Publishing Authors By Initials

CA Ramos,TA Bowman,NC Boles,AA Merchant,Y Zheng,I Parra,SA Fuqua,CA Shaw,MA Goodell,

For similar genetic phenomena: variation (genetics) research abstracts see: genetic phenomena: variation (genetics) research

PUBMED ID PMID:

MEDLINE DATE:

Evidence for diversity in transcriptional profiles of single hematopoietic stem cells. Journal Published:

PUBLICATION TYPE: Validation Studies

Journal: PLoS genetics

VOLUME: 2

Page Numbers: e159

Journal Abbreviation: PLoS Genet.

ISSN: 1553-7404

DAY: 9

MONTH: 08

YEAR: 2006

Evidence for diversity in transcriptional profiles of single hematopoietic stem cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101239074

Evidence for diversity in transcriptional profiles of single hematopoietic stem cells. Keywords Mesh Terms:

KEYWORDS: Variation (Genetics)

MESH TERMS: methods

Chemical & Substance for Abstract: Evidence for diversity in transcriptional profiles of single hematopoietic stem cells. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Evidence for diversity in transcriptional profiles of single hematopoietic stem cells.

AFFILIATION: Department of Medicine, Baylor College of Medicine, Houston, Texas, United States of America.

Country: United States

AGENCY: United States NIDDK

GRANT: UO1 DK63588

ACRONYM: DK

MEDLINETA: PLoS Genet

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Evidence for diversity in transcriptional profiles of single hematopoietic stem cells Related Publications

• Aging hematopoietic stem cells decline in function and exhibit epigenetic dysregulation.
• An Argument against a Role for Oct4 in Somatic Stem Cells.
• An agar diffusion comparison of the antimicrobial effect of calcium hydroxide at five different concentrations with three different vehicles.
• CD150- side population cells represent a functionally distinct population of long-term hematopoietic stem cells.
• Disclosure of psychiatric records.
• Effect of analgesics on the intensity of pain experienced; preliminary report.
• Effect of dentin thickness on root surface temperature of teeth undergoing ultrasonic removal of posts.
• Effect of shortened irrigation times with 17% ethylene diamine tetra-acetic acid on smear layer removal after rotary canal instrumentation.
• Evidence for diversity in transcriptional profiles of single hematopoietic stem cells.
• Evidence on the genesis of certain common nasal disorders.
• Experimental studies on the nature of hyperalgesia.
• Fluid flow evaluation of Fuji Triage and gray and white ProRoot mineral trioxide aggregate intraorifice barriers.
• Hematopoietic stem cell aging: wrinkles in stem cell potential.
• In Vitro Comparison of Coronal Microleakage between Resilon Alone and Gutta-Percha with a Glass-ionomer Intraorifice Barrier Using a Fluid Filtration Model.
• Observations on the spread of pain.
• Pluripotentiality and conditional transgene regulation in human embryonic stem cells expressing insulated tetracycline-ON transactivator.
• Post-operative blindness, with complete recovery, in a patient with sickle-cell anaemia.
• Promiscuous Expression of H2B-GFP Transgene in Hematopoietic Stem Cells.
• Research glut and information famine: making research evidence more useful for policymakers.
• Response: The CD150high compartment is not the exclusive reservoir of LT-HSCs within the bone marrow.
• Setting expansion of gray and white mineral trioxide aggregate and portland cement.
• Sexuality in chronic lung disease.
• Stem Cell Identification and Sorting Using the Hoechst 33342 Side Population (SP).
• Studies on pain; an investigation of some quantitative aspects of the dol scale of pain intensity.
• The desire to die at home.
• The p47 GTPase Lrg-47 (Irgm1) Links Host Defense and Hematopoietic Stem Cell Proliferation.