Evidence for a stem cell hierarchy in the adult human breast.

Evidence for a stem cell hierarchy in the adult human breast. Research Abstract Details 

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Evidence for a stem cell hierarchy in the adult human breast. Abstract Text:

Villadsen,Agla J Fridriksdottir,Lone ,Thorarinn Gudjonsson,Fritz Rank,Mark A LaBarge,Mina J Bissell,Ole W Petersen,

Cellular pathways that contribute to adult human mammary gland architecture and lineages have not been previously described. In this study, we identify a candidate stem cell niche in ducts and zones containing progenitor cells in lobules. Putative stem cells residing in ducts were essentially quiescent, whereas the progenitor cells in the lobules were more likely to be actively dividing. Cells from ducts and lobules collected under the microscope were functionally characterized by colony formation on tissue culture plastic, mammosphere formation in suspension culture, and morphogenesis in laminin-rich extracellular matrix gels. Staining for the lineage markers keratins K14 and K19 further revealed multipotent cells in the stem cell zone and three lineage-restricted cell types outside this zone. Multiparameter cell sorting and functional characterization with reference to anatomical sites in situ confirmed this pattern. The proposal that the four cell types are indeed constituents of an as of yet undescribed stem cell hierarchy was assessed in long-term cultures in which senescence was bypassed. These findings identify an adult human breast ductal stem cell activity and its earliest descendants.

Evidence for a stem cell hierarchy in the adult human breast. Publishing Authors By Initials

R Villadsen,AJ Fridriksdottir,L ,T Gudjonsson,F Rank,MA LaBarge,MJ Bissell,OW Petersen,

For similar investigative techniques: genetic techniques: gene transfer techniques: transduction, genetic research abstracts see: investigative techniques: genetic techniques: gene transfer techniques: transduction, genetic research

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Evidence for a stem cell hierarchy in the adult human breast. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of cell biology

VOLUME: 177

Page Numbers: 87-101

Journal Abbreviation:

ISSN: 0021-9525

DAY: 9

MONTH: Apr

YEAR: 2007

Evidence for a stem cell hierarchy in the adult human breast. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 375356

Evidence for a stem cell hierarchy in the adult human breast. Keywords Mesh Terms:

KEYWORDS: Transduction, Genetic

MESH TERMS: metabolism

Chemical & Substance for Abstract: Evidence for a stem cell hierarchy in the adult human breast. Information

Substance Name: Keratins

Registry Number: 68238-35-7

Grant and Affiliation Information for Evidence for a stem cell hierarchy in the adult human breast.

AFFILIATION: Department of Cellular and Molecular Medicine, Faculty of Health Sciences, and Zoophysiological Laboratory, University of Copenhagen, and Department of Pathology, State University Hospital, Copenhagen, Denmark.

Country: United States

AGENCY: United States NCI

GRANT: CA-64786

ACRONYM: CA

MEDLINETA: J Cell Biol

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