Even illumination in total internal reflection fluorescence microscopy using laser light.

Even illumination in total internal reflection fluorescence microscopy using laser light. Research Abstract Details 

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Even illumination in total internal reflection fluorescence microscopy using laser light. Abstract Text:

R Fiolka,Y Belyaev,H Ewers,A Stemmer,R Fiolka,Y Belyaev,H Ewers,A Stemmer,

In modern fluorescence microscopy, lasers are a widely used source of light, both for imaging in total internal reflection and epi-illumination modes. In wide-field imaging, scattering of highly coherent laser light due to imperfections in the light path typically leads to nonuniform illumination of the specimen, compromising image analysis. We report the design and construction of an objective-launch total internal reflection fluorescence microscopy system with excellent evenness of specimen illumination achieved by azimuthal rotation of the incoming illuminating laser beam. The system allows quick and precise changes of the incidence angle of the laser beam and thus can also be used in an epifluorescence mode. Microsc. Res. Tech., 2008. (c) 2007 Wiley-Liss, Inc.

Even illumination in total internal reflection fluorescence microscopy using laser light. Publishing Authors By Initials

R Fiolka,Y Belyaev,H Ewers,A Stemmer,R Fiolka,Y Belyaev,H Ewers,A Stemmer,

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Even illumination in total internal reflection fluorescence microscopy using laser light. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Microscopy research and technique

VOLUME: 71

Page Numbers: 45-50

Journal Abbreviation: Microsc. Res. Tech.

ISSN: 1059-910X

DAY: 24

MONTH: Jan

YEAR: 2008

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LANGUAGE: eng

NlmUniqueID: 9203012

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AFFILIATION: Nanotechnology Group, ETH Zurich, Tannenstrasse 3, CH-8092 Zurich, Switzerland.

Country: United States

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MEDLINETA: Microsc Res Tech

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