Evaluation of the anti-LipL32 monoclonal antibodies potential for use in leptospirosis immunodiagnostic tests.

Evaluation of the anti-LipL32 monoclonal antibodies potential for use in leptospirosis immunodiagnostic tests. Research Abstract Details 

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Evaluation of the anti-LipL32 monoclonal antibodies potential for use in leptospirosis immunodiagnostic tests. Abstract Text:

M L Coutinho,F A Vasconcellos,C P H Fernandes,N Seyffert,F K Seixas,A I Ko,O A Dellagostin,J A G Aleixo,

LipL32 is the major lipoprotein in the membrane of pathogenic leptospira. In this work, we report on the production of monoclonal antibodies (MAbs) against recombinant LipL32 (rLipL32) and on the evaluation of their potential for use as reagents in diagnostic tests for leptospirosis. The MAbs were all of the IgG(2b) isotype and reacted specifically with native LipL32 in pathogenic serovars only. MAbs reacted in the same region of the rLipL32 molecule and their affinity constant was between 5x10(7) M(-1) and 6x10(6) M(-1). These results suggest that although the MAbs cannot be used together, they are well suited for diagnostic tests of leptospirosis based on LipL32 detection.

Evaluation of the anti-LipL32 monoclonal antibodies potential for use in leptospirosis immunodiagnostic tests. Publishing Authors By Initials

ML Coutinho,FA Vasconcellos,CP Fernandes,N Seyffert,FK Seixas,AI Ko,OA Dellagostin,JA Aleixo,

For similar lipids: lipoproteins research abstracts see: lipids: lipoproteins research

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Evaluation of the anti-LipL32 monoclonal antibodies potential for use in leptospirosis immunodiagnostic tests. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of immunoassay & immunochemistry

VOLUME: 28

Page Numbers: 279-88

Journal Abbreviation:

ISSN: 1532-1819

DAY: 3

MONTH: 12

YEAR: 2007

Evaluation of the anti-LipL32 monoclonal antibodies potential for use in leptospirosis immunodiagnostic tests. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100963688

Evaluation of the anti-LipL32 monoclonal antibodies potential for use in leptospirosis immunodiagnostic tests. Keywords Mesh Terms:

KEYWORDS: Lipoproteins

MESH TERMS: immunology

Chemical & Substance for Abstract: Evaluation of the anti-LipL32 monoclonal antibodies potential for use in leptospirosis immunodiagnostic tests. Information

Substance Name: Lipoproteins

Registry Number: 0

Grant and Affiliation Information for Evaluation of the anti-LipL32 monoclonal antibodies potential for use in leptospirosis immunodiagnostic tests.

AFFILIATION: Center for Biotechnology, Federal University of Pelotas, Rio Grande do Sul, Brazil.

Country: United States

AGENCY: United States FIC

GRANT: TW00919

ACRONYM: TW

MEDLINETA: J Immunoassay Immunochem

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