Evaluation of RNA markers for early detection of cervical neoplasia in exfoliated cervical cells.

Evaluation of RNA markers for early detection of cervical neoplasia in exfoliated cervical cells. Research Abstract Details 

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Evaluation of RNA markers for early detection of cervical neoplasia in exfoliated cervical cells. Abstract Text:

Martin Steinau,Mangalathu S Rajeevan,Daisy R Lee,Mack T Ruffin,Ira R Horowitz,Lisa C Flowers,Talaat Tadros,George Birdsong,Mujtaba Husain,David C Kmak,Garry M Longton,Suzanne D Vernon,Elizabeth R Unger,

Numerous molecular biomarkers have been suggested for early detection of cervical cancer, but their usefulness in routinely collected exfoliated cells remains uncertain. We used quantitative reverse transcription-PCR to evaluate expression of 40 candidate genes as markers for high-grade cervical intraepithelial neoplasia (CIN) in exfoliated cervical cells collected at the time of colposcopy. Samples from the 93 women with CIN3 or cancer were compared with those from 186 women without disease matched (1:2) for age, race, and high-risk human papillomavirus status. Normalized threshold cycles (C(t)) for each gene were analyzed by receiver operating characteristics to determine their diagnostic performance in a split sample validation approach. Six markers were confirmed by an area under the curve >0.6 in both sample sets: claudin 1 (0.75), minichromosome maintenance deficient 5 (0.71) and 7 (0.64), cell division cycle 6 homologue (0.71), antigen identified by monoclonal antibody Ki-67 (0.66), and SHC SH2-domain binding protein 1 (0.61). The sensitivity for individual markers was relatively low and a combination of five genes to a panel resulted in 60% sensitivity with 76% specificity, not positively increasing this performance. Although the results did not indicate superiority of RNA markers for cervical cancer screening, their performance in detecting disease in women referred for colposcopy suggests that the genes and pathways they highlight could be useful in alternative detection formats or in combination with other screening indicators.

Evaluation of RNA markers for early detection of cervical neoplasia in exfoliated cervical cells. Publishing Authors By Initials

M Steinau,MS Rajeevan,DR Lee,MT Ruffin,IR Horowitz,LC Flowers,T Tadros,G Birdsong,M Husain,DC Kmak,GM Longton,SD Vernon,ER Unger,

For similar population characteristics: population: urban population research abstracts see: population characteristics: population: urban population research

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Evaluation of RNA markers for early detection of cervical neoplasia in exfoliated cervical cells. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Cancer epidemiology, biomarkers & prevention : a p

VOLUME: 16

Page Numbers: 295-301

Journal Abbreviation: Cancer Epidemiol. Biomarkers P

ISSN: 1055-9965

DAY: 3

MONTH: Feb

YEAR: 2007

Evaluation of RNA markers for early detection of cervical neoplasia in exfoliated cervical cells. Information

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LANGUAGE: eng

NlmUniqueID: 9200608

Evaluation of RNA markers for early detection of cervical neoplasia in exfoliated cervical cells. Keywords Mesh Terms:

KEYWORDS: Urban Population

MESH TERMS: analysis

Chemical & Substance for Abstract: Evaluation of RNA markers for early detection of cervical neoplasia in exfoliated cervical cells. Information

Substance Name: RNA

Registry Number: 63231-63-0

Grant and Affiliation Information for Evaluation of RNA markers for early detection of cervical neoplasia in exfoliated cervical cells.

AFFILIATION: Division of Viral and Rickettsial Disease, Viral Exanthems and Herpesvirus Branch, Centers for Disease Control and Prevention, 1600 Clifton Road, MS G-41, Atlanta, GA 30333, USA. MSteinau@cdc.gov

Country: United States

AGENCY: United States NCI

GRANT: Y1-CN-5005-01

ACRONYM: CN

MEDLINETA: Cancer Epidemiol Biomarkers Pr

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