Evaluation of colors in green mutants isolated from purple bacteria as a host for colorimetric whole-cell biosensors.

Evaluation of colors in green mutants isolated from purple bacteria as a host for colorimetric whole-cell biosensors. Research Abstract Details 

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Evaluation of colors in green mutants isolated from purple bacteria as a host for colorimetric whole-cell biosensors. Abstract Text:

Kazuyuki Yoshida,Daiki Yoshioka,Koichi Inoue,Shinichi Takaichi,Isamu Maeda,

The change in carotenoid-based bacterial color from yellow to red can be applied to whole-cell biosensors. We generated several green mutants to emphasize the color change in such biosensors. The blue-green crtI-deleted mutant, Rhodopseudomonas palustris no.711, accumulated the colorless carotenoid precursor, phytoene. Green Rhodovulum sulfidophilum M31 accumulated neurosporene, a downstream product of phytoene. Another green mutant, Rhodobacter sphaeroides Ga, accumulated neurosporene and chloroxanthin, which are both downstream products of phytoene. All green mutants accumulated bacteriochlorophyll a. Photosynthetic membrane obtained from the green mutants all exhibited decreased absorption of wavelength range at 510-570 nm. Therefore, these indicate that the greenish bacterial colors were mainly caused by the existence of bacteriochlorophyll a and the changes in carotenoid composition in photosynthetic membrane. The colors of the green mutants and their wild-type strains were plotted in the CIE-L*a*b* color space, and the color difference (DeltaE*ab) values between a green mutant and its wild type were calculated. DeltaE*ab values were higher in the green mutants than in Rdv. sulfidophilum CDM2, the yellowish host strain of reported biosensors. These data indicate that change in bacterial color from green to red is more distinguishable than that from yellow to red as a reporter signal of carotenoid-based whole-cell biosensors.

Evaluation of colors in green mutants isolated from purple bacteria as a host for colorimetric whole-cell biosensors. Publishing Authors By Initials

K Yoshida,D Yoshioka,K Inoue,S Takaichi,I Maeda,

For similar bacteria: gram-negative bacteria: rhodovulum research abstracts see: bacteria: gram-negative bacteria: rhodovulum research

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Evaluation of colors in green mutants isolated from purple bacteria as a host for colorimetric whole-cell biosensors. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Applied microbiology and biotechnology

VOLUME: 76

Page Numbers: 1043-50

Journal Abbreviation: Appl. Microbiol. Biotechnol.

ISSN: 0175-7598

DAY: 4

MONTH: 07

YEAR: 2007

Evaluation of colors in green mutants isolated from purple bacteria as a host for colorimetric whole-cell biosensors. Information

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LANGUAGE: eng

NlmUniqueID: 8406612

Evaluation of colors in green mutants isolated from purple bacteria as a host for colorimetric whole-cell biosensors. Keywords Mesh Terms:

KEYWORDS: Rhodovulum

MESH TERMS: metabolism

Chemical & Substance for Abstract: Evaluation of colors in green mutants isolated from purple bacteria as a host for colorimetric whole-cell biosensors. Information

Substance Name: Carotenoids

Registry Number: 36-88-4

Grant and Affiliation Information for Evaluation of colors in green mutants isolated from purple bacteria as a host for colorimetric whole-cell biosensors.

AFFILIATION: Faculty of Agriculture, Utsunomiya University, Minemachi, Utsunomiya 321-8505, Japan.

Country: Germany

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MEDLINETA: Appl Microbiol Biotechnol

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