Evaluation of a cytolethal distending toxin (cdt) gene-based species-specific multiplex PCR assay for the identification of Campylobacter strains isolated from poultry in Thailand.

Evaluation of a cytolethal distending toxin (cdt) gene-based species-specific multiplex PCR assay for the identification of Campylobacter strains isolated from poultry in Thailand. Research Abstract Details 

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Evaluation of a cytolethal distending toxin (cdt) gene-based species-specific multiplex PCR assay for the identification of Campylobacter strains isolated from poultry in Thailand. Abstract Text:

Worada Samosornsuk,Masahiro Asakura,Emi Yoshida,Takashi Taguchi,Kazuhiko Nishimura,Boonchuay Eampokalap,Vongsavanh Phongsisay,Wanpen Chaicumpa,Shinji Yamasaki,Worada Samosornsuk,Masahiro Asakura,Emi Yoshida,Takashi Taguchi,Kazuhiko Nishimura,Boonchuay Eampokalap,Vongsavanh Phongsisay,Wanpen Chaicumpa,Shinji Yamasaki,

We have recently developed a cytolethal distending toxin (cdt) gene-based species-specific multiplex PCR assay for identifying Campylobacter jejuni, C. coli and C. fetus. In the present study, the applicability of this assay was evaluated with 34 Campylobacter-like organisms isolated from poultry in Thailand for species identification and was compared with other assays including API Campy, 16S rRNA gene sequence, and hippuricase (hipO) gene detection. Of the 34 strains analyzed, 20, 10 and 1 were identified as C. jejuni, C. coli, and Arcobacter cryaerophilus, respectively, and 3 could not be identified by API Campy. However, 16S rRNA gene analysis, showed that all 34 strains are C. jejuni/coli. To discriminate between these 2 species, the hipO gene, which is specifically present in C. jejuni, was examined by PCR and was detected in 20 strains, which were identified as C. jejuni by API Campy but not in the remaining 14 strains. Collective results indicated that 20 strains were C. jejuni whereas the 14 strains were C. coli. When the cdt gene-based multiplex PCR was employed, however, 19, 20 and 19 strains were identified as C. jejuni while 13, 14 and 13 were identified as C. coli by the cdtA, cdtB and cdtC gene-based multiplex PCR, respectively. Pulsed-field gel electrophoresis revealed that C. jejuni and C. coli strains analyzed are genetically diverse. Taken together, these data suggest that the cdt gene-based multiplex PCR, particularly cdtB gene-based multiplex PCR, is a simple, rapid and reliable method for identifying the species of Campylobacter strains.

Evaluation of a cytolethal distending toxin (cdt) gene-based species-specific multiplex PCR assay for the identification of Campylobacter strains isolated from poultry in Thailand. Publishing Authors By Initials

W Samosornsuk,M Asakura,E Yoshida,T Taguchi,K Nishimura,B Eampokalap,V Phongsisay,W Chaicumpa,S Yamasaki,W Samosornsuk,M Asakura,E Yoshida,T Taguchi,K Nishimura,B Eampokalap,V Phongsisay,W Chaicumpa,S Yamasaki,

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Evaluation of a cytolethal distending toxin (cdt) gene-based species-specific multiplex PCR assay for the identification of Campylobacter strains isolated from poultry in Thailand. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Microbiology and immunology

VOLUME: 51

Page Numbers: 909-17

Journal Abbreviation: Microbiol. Immunol.

ISSN: 0385-5600

DAY: 26

MONTH: 09

YEAR: 2007

Evaluation of a cytolethal distending toxin (cdt) gene-based species-specific multiplex PCR assay for the identification of Campylobacter strains isolated from poultry in Thailand. Information

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LANGUAGE: eng

NlmUniqueID: 7703966

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Grant and Affiliation Information for Evaluation of a cytolethal distending toxin (cdt) gene-based species-specific multiplex PCR assay for the identification of Campylobacter strains isolated from poultry in Thailand.

AFFILIATION: Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka, Japan.

Country: Japan

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MEDLINETA: Microbiol Immunol

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