Evaluation and quantification of nuclear DNA from human telogen hairs*.

Evaluation and quantification of nuclear DNA from human telogen hairs*. Research Abstract Details 

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Evaluation and quantification of nuclear DNA from human telogen hairs*. Abstract Text:

Nuclear DNA was extracted from human telogen hairs from 60 individuals. Six to nine hairs from each individual were individually extracted. The amount of DNA recovered from each individual varied greatly, and most samples yielded a quantity of 550 pg or less per hair. A selective extraction buffer was used to remove epithelial cell DNA and the amount of exogenous DNA was determined. DNA was also quantified by real time PCR using three different sized amplicons targeting an Alu sequence. The results were used to determine the state of degradation of the extracted DNA. Different quantities of sample (<100pg, 100-500pg, >500pg) were amplified with the Miniplex kits to determine the minimum DNA template required for successful amplification. DNA recovered from hair showed degradation; however, partial profiles were obtained for those samples containing at least 60 pg using MiniSTRs.

Evaluation and quantification of nuclear DNA from human telogen hairs*. Publishing Authors By Initials

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Evaluation and quantification of nuclear DNA from human telogen hairs*. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of forensic sciences

VOLUME: 53

Page Numbers: 853-7

Journal Abbreviation: J. Forensic Sci.

ISSN: 1556-4029

DAY: 21

MONTH: Jul

YEAR: 2008

Evaluation and quantification of nuclear DNA from human telogen hairs*. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Evaluation and quantification of nuclear DNA from human telogen hairs*.

AFFILIATION: Department of Chemistry and Biochemistry, Florida International University, University Park, 11200 SW 8th Street, Miami, FL 33199, U.S.A.

Country: United States

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MEDLINETA: J Forensic Sci

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