Evaluating measurement uncertainty in the microbiological assay of vancomycin from methodology validation data.

Evaluating measurement uncertainty in the microbiological assay of vancomycin from methodology validation data. Research Abstract Details 

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Evaluating measurement uncertainty in the microbiological assay of vancomycin from methodology validation data. Abstract Text:

Felipe Rebello ,Telma Mary Kaneko,Terezinha de Jesus Andreoli Pinto,

Methodology validation and measurement uncertainty estimation are fundamental to obtain reliable results. The microbiological methods are widely used to determine antibiotic assay, as they permit evaluation of the analyzed antibiotic activity. A microbiological assay of vancomycin was performed with adoption of experimental design 5 x 1 (interpolation in 5-point standard curve assay) with final concentrations from 6.4 to 15.6 microg/mL (standards) and 10.0 microg/mL (sample). Bacillus subtitlis (ATCC 6633) was the microorganism used, with antibiotic medium No. 8 as base layer and inoculated layer. The plates were incubated at 37 degrees C for 18 h. The method adopted for the microbiological assay of vancomycin through agar diffusion was validated according to statistic results demonstrated for suitability of the method concerning linearity, precision, and accuracy. The estimated relative expanded uncertainty (4.3%) was considered adequate for this method purpose.

Evaluating measurement uncertainty in the microbiological assay of vancomycin from methodology validation data. Publishing Authors By Initials

FR ,TM Kaneko,Tde Pinto,

For similar carbohydrates: glycoconjugates: glycopeptides: vancomycin research abstracts see: carbohydrates: glycoconjugates: glycopeptides: vancomycin research

PUBMED ID PMID:

MEDLINE DATE: 2007 Sep-Oct

Evaluating measurement uncertainty in the microbiological assay of vancomycin from methodology validation data. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of AOAC International

VOLUME: 90

Page Numbers: 1383-6

Journal Abbreviation:

ISSN: 1060-3271

DAY: 28

MONTH: 11

YEAR: 2007

Evaluating measurement uncertainty in the microbiological assay of vancomycin from methodology validation data. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9215446

Evaluating measurement uncertainty in the microbiological assay of vancomycin from methodology validation data. Keywords Mesh Terms:

KEYWORDS: Vancomycin

MESH TERMS: analysis

Chemical & Substance for Abstract: Evaluating measurement uncertainty in the microbiological assay of vancomycin from methodology validation data. Information

Substance Name: Vancomycin

Registry Number: 1404-90-6

Grant and Affiliation Information for Evaluating measurement uncertainty in the microbiological assay of vancomycin from methodology validation data.

AFFILIATION: University of São Paulo, Faculty of Pharmaceutical Science, Department of Pharmacy, São Paulo, Brazil. feliperl@usp.br

Country: United States

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MEDLINETA: J AOAC Int

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