[Eukaryotic expression and application of human CD226 Ig fusion protein containing 3C protease-restricted site]

[Eukaryotic expression and application of human CD226 Ig fusion protein containing 3C protease-restricted site] Research Abstract Details 

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[Eukaryotic expression and application of human CD226 Ig fusion protein containing 3C protease-restricted site] Abstract Text:

Xin-Hai Zhang,Kun Yang,Wei Jia,Wei-Ming Ouyang,Ying Liu,Xiao-Guang Xu,Qi Li,Bo-Quan Jin,

AIM: To construct, express and characterize the eukaryotic expression vector of encoding human CD226 (PTA1) extracellular region Ig fusion protein gene containing 3C protease-restricted site. METHODS: The gene fragment encoding extracellular region of human CD226 was cloned into the eukaryotic expression vector p-3C-Ig containing 3C protease-restricted site and human Ig Fc fragment gene. After sequencing, the vector was transfected into COS7 cells, and the expressed molecule was purified by affinity chromatography. Finally, the product was characterized by immunofluorescent staining and 3C protease digestion. RESULTS: After expression and purification, the Ig fusion protein could bind effectively to the CD226 ligand expressed on ECV304 cells. The Fc fragment could also be cut off by 3C protease, so that the CD226 extracellular fragment was obtained. CONCLUSION: The extracellular region of human CD226 Ig fusion protein with 3C protease-restricted site is expressed successfully in COS-7 cells, which lays the foundation for the structural and functional study of this molecule.

[Eukaryotic expression and application of human CD226 Ig fusion protein containing 3C protease-restricted site] Publishing Authors By Initials

XH Zhang,K Yang,W Jia,WM Ouyang,Y Liu,XG Xu,Q Li,BQ Jin,

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[Eukaryotic expression and application of human CD226 Ig fusion protein containing 3C protease-restricted site] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese jour

VOLUME: 19

Page Numbers: 288-90

Journal Abbreviation: Xi Bao Yu Fen Zi Mian Yi Xue Z

ISSN: 1007-8738

DAY: 24

MONTH: May

YEAR: 2003

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LANGUAGE: chi

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AFFILIATION: Department of Immunology, Fourth Military Medical University, Xi'an 710032, China.

Country: China

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MEDLINETA: Xi Bao Yu Fen Zi Mian Yi Xue Z

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